验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, Jurkat cells, human heart tissue, human kidney tissue, HEK-293T cells |
Positive IHC detected in | mouse cerebellum tissue, human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HT-1080 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
13441-1-AP targets SFRS17A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
经测试反应性 | human |
免疫原 | SFRS17A fusion protein Ag4252 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | splicing factor, arginine/serine-rich 17A |
别名 | CXYorf3, B-lymphocyte antigen, A-kinase anchor protein 17A, AKAP-17A, AKAP17A |
计算分子量 | 695 aa, 81 kDa |
观测分子量 | 81 kDa |
GenBank蛋白编号 | BC028151 |
基因名称 | SFRS17A |
Gene ID (NCBI) | 8227 |
RRID | AB_2184937 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q02040 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
Product Specific Protocols | |
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WB protocol for SFRS17A antibody 13441-1-AP | Download protocol |
IHC protocol for SFRS17A antibody 13441-1-AP | Download protocol |
IF protocol for SFRS17A antibody 13441-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |