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  • Featured Product
  • KD/KO Validated

SFRS11 Polyclonal antibody

SFRS11 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 26751-1-AP

Print datasheet

Synonyms

p54, SFRS11, SRSF11

验证数据展示

  • IHC staining of human breast cancer using 26751-1-AP

    IHC staining of human breast cancer using 26751-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26751-1-AP (SFRS11 antibody) at dilution of 1:1000 (under 10x lens).

    Ready-to-use IHC kits available with SRSF11 antibody included
  • IHC staining of human breast cancer using 26751-1-AP

    IHC staining of human breast cancer using 26751-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26751-1-AP (SFRS11 antibody) at dilution of 1:1000 (under 40x lens).

    Ready-to-use IHC kits available with SRSF11 antibody included
  • IHC staining of rat colon using 26751-1-AP

    IHC staining of rat colon using 26751-1-AP

    Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 26751-1-AP (SFRS11 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SRSF11 antibody included
  • IHC staining of rat colon using 26751-1-AP

    IHC staining of rat colon using 26751-1-AP

    Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 26751-1-AP (SFRS11 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SRSF11 antibody included
  • WB analysis of HEK-293T using 26751-1-AP

    WB analysis of HEK-293T using 26751-1-AP

    WB result of SFRS11 antibody (26751-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-SFRS11 transfected HEK-293T cells.

  • WB analysis using 26751-1-AP

    WB analysis using 26751-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26751-1-AP (SFRS11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHEK-293T cells
Positive IHC detected inhuman breast cancer tissue, rat colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy SRSF11 Ready-To-Use IHC Kit. SRSF11 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26751-1-AP targets SFRS11 in WB, IHC, ELISA applications and shows reactivity with human, rat samples.

Tested Applications IHC, WB, ELISA
Tested Reactivity human, rat
Immunogen SFRS11 fusion protein Ag25015 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name splicing factor, arginine/serine-rich 11
Synonyms p54, SFRS11, SRSF11
Calculated Molecular Weight 483 aa, 53 kDa
Observed Molecular Weight 68 kDa
GenBank Accession NumberBC040436
Gene Symbol SFRS11
Gene ID (NCBI) 9295
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ05519
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SFRS11 antibody 26751-1-APDownload protocol
IHC protocol for SFRS11 antibody 26751-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols