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- KD/KO Validated
SFRP4 Polyclonal antibody
SFRP4 Polyclonal Antibody for IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, rat and More (1)
Applications
IHC, IP, WB, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, HeLa cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human breast cancer tissue, human skin cancer tissue, rat testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
15328-1-AP targets SFRP4 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, rat samples.
| Tested Applications | IHC, IP, WB, ELISA |
| Cited Applications | WB, IF, IHC |
| Tested Reactivity | human, rat |
| Cited Reactivity | human, mouse |
| Immunogen | SFRP4 fusion protein Ag6909 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | secreted frizzled-related protein 4 |
| Synonyms | FRP 4, FRPHE, sFRP 4, SFRP4 |
| Calculated Molecular Weight | 40 kDa |
| Observed Molecular Weight | 41-51 kDa, 55 kDa |
| GenBank Accession Number | BC058911 |
| Gene Symbol | SFRP4 |
| Gene ID (NCBI) | 6424 |
| RRID | AB_2187099 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q6FHJ7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Secreted frizzled-related protein 4 (SFRP4) is a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRP4 functions as a suppressor of tumor growth via inhibition of the Wnt signaling pathway.Downregulation or deletion of SFRP4 has been reported in several cancers and cell lines such as endometrial cancer, ovarian cancer, lung cancer,and others. This antibody got 41-51 kDa and 55 kDa bands in western blotting maybe due to differential posttranslational protein modification.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SFRP4 antibody 15328-1-AP | Download protocol |
| IHC protocol for SFRP4 antibody 15328-1-AP | Download protocol |
| IP protocol for SFRP4 antibody 15328-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun Myofibroblast transcriptome indicates SFRP2hi fibroblast progenitors in systemic sclerosis skin. | ||
Sci Rep Expression profiling of prostate cancer tissue delineates genes associated with recurrence after prostatectomy. | ||
Cell Physiol Biochem Differential Patterns of Secreted Frizzled-Related Protein 4 (SFRP4) in Adipocyte Differentiation: Adipose Depot Specificity.
| ||
Gene Ther Effect of nanoparticle-mediated delivery of SFRP4 siRNA for treating Dupuytren disease.
| ||
Endocrinology SFRP4 Is a Negative Regulator of Ovarian Follicle Development and Female Fertility.
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