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SF4 Monoclonal antibody

SF4 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1G9D8

Cat No : 68319-1-Ig

Print datasheet

Synonyms

DKFZp434E2216, F23858, RBP, RNA binding protein RBP, SF4, splicing factor 4

验证数据展示

  • WB analysis using 68319-1-Ig

    WB analysis using 68319-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68319-1-Ig (SF4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

  • WB analysis of NCCIT using 68319-1-Ig

    WB analysis of NCCIT using 68319-1-Ig

    NCCIT cells were subjected to SDS PAGE followed by western blot with 68319-1-Ig (SF4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inU2OS cells, NCCIT cells, A549 cells, LNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, MOLT-4 cells, K-562 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68319-1-Ig targets SF4 in WB, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, ELISA
Tested Reactivity Human
Immunogen SF4 fusion protein Ag8014 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name splicing factor 4
Synonyms DKFZp434E2216, F23858, RBP, RNA binding protein RBP, SF4, splicing factor 4
Calculated Molecular Weight 72 kDa
Observed Molecular Weight72 kDa
GenBank Accession NumberBC002986
Gene Symbol SF4
Gene ID (NCBI) 57794
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ8IWZ8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SF4 antibody 68319-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols