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SF4 Polyclonal antibody

SF4 Polyclonal Antibody for IF/ICC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 15614-1-AP

Print datasheet

Synonyms

F23858, RBP, RNA binding protein RBP, SF4, splicing factor 4

验证数据展示

  • WB analysis of HEK-293 using 15614-1-AP

    WB analysis of HEK-293 using 15614-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 15614-1-AP (SF4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 15614-1-AP

    IHC staining of human testis using 15614-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 15614-1-AP (SF4 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human testis using 15614-1-AP

    IHC staining of human testis using 15614-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 15614-1-AP (SF4 antibody) at dilution of 1:100 (under 40x lens).

  • IF Staining of HepG2 using 15614-1-AP

    IF Staining of HepG2 using 15614-1-AP

    Immunofluorescent analysis of () fixed HepG2 cells using 15614-1-AP (SF4 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

经过测试的应用

Positive WB detected inHEK-293 cells, mouse testis tissue
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15614-1-AP targets SF4 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, IHC, WB, ELISA
Tested Reactivity human, mouse, rat
Immunogen SF4 fusion protein Ag8006 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name splicing factor 4
Synonyms F23858, RBP, RNA binding protein RBP, SF4, splicing factor 4
Calculated Molecular Weight 72 kDa
Observed Molecular Weight72 kDa
GenBank Accession NumberBC002986
Gene Symbol SF4
Gene ID (NCBI) 57794
RRIDAB_10859818
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8IWZ8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SF4 antibody 15614-1-APDownload protocol
IHC protocol for SF4 antibody 15614-1-APDownload protocol
IF protocol for SF4 antibody 15614-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols