验证数据展示
产品信息
12379-1-PBS targets SF3B14 in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, Indirect ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag3045 Product name: Recombinant human SF3B14 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-125 aa of BC015463 Sequence: MAMQAAKRANIRLPPEVNRILYIRNLPYKITAEEMYDIFGKYGPIRQIRVGNTPETRGTAYVVYEDIFDAKNACDHLSGFNVCNRYLVVLYYNANRAFQKMDTKKKEEQLKLLKEKYGINTDPPK 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | splicing factor 3B, 14 kDa subunit |
| 别名 | |
| 计算分子量 | 14 kDa |
| 观测分子量 | 14 kDa |
| GenBank蛋白编号 | BC015463 |
| 基因名称 | SF3B14 |
| Gene ID (NCBI) | 51639 |
| RRID | AB_2186517 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9Y3B4 |
| 储存缓冲液 | PBS only, pH 7.3. |
| 储存条件 | Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C |
背景介绍
Pre-mRNA splicing by the human spliceosome requires the sequential recognition by RNA and protein factors of the 5′ and 3′ splice sites,the branch region and the polypyrimidine tract. This sequence is characterized by the stepwise association of the U1, U2, and U4/U5/U6 snRNP particles [PMID:9476892]. Human p14 (SF3b14), a component of the spliceosomal U2 snRNP, interacts directly with the pre-mRNA branch adenosine within the context of the bulged duplex formed between the pre-mRNA branch region and U2 snRNA. This association occurs early in spliceosome assembly and persists within the fully assembled spliceosome [PMID:21062891].
