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验证数据展示

  • WB analysis of HL-60 using 25814-1-AP

    WB analysis of HL-60 using 25814-1-AP

    HL-60 cells were subjected to SDS PAGE followed by western blot with 25814-1-AP (SETMAR Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IP experiment of HL-60 using 25814-1-AP

    IP experiment of HL-60 using 25814-1-AP

    IP result of anti-SETMAR (IP:25814-1-AP, 4ug; Detection:25814-1-AP 1:500) with HL-60 cells lysate 1600ug.

  • WB analysis using 25814-1-AP

    WB analysis using 25814-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25814-1-AP (SETMAR antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

SETMAR Polyclonal antibody

SETMAR Polyclonal Antibody for WB, IP, ELISA
Cat No. 25814-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IP, ELISA

别名

HsMar1, METNASE, SETMAR

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHL-60 cells, L02 cells, HEK-293T cells, Jurkat cells
Positive IP detected inHL-60 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

25814-1-AP targets SETMAR in WB, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IP, ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性human
免疫原

CatNo: Ag22938

Product name: Recombinant human SETMAR protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 80-429 aa of BC008931

Sequence: KRLTLETMKMMLDKKQIRAIFLFEFKMGRKAAETTRNINNAFGPGTANERTVQWWFKKFCKGDESLEDEERSGRPSEVDNDQLRAIIEADPLTTTREVAEELNVNHSTVVRHLKQIGKVKKLDKWVPHELTENQKNRRFEVSSSLILRNHNEPFLDRIVTCDEKWILYDNRRRSAQWLDQEEAPKHFPKPILHPKKVMVTIWWSAAGLIHYSFLNPGETITSEKYAQEIDEMNQKLQRLQLALVNRKGPILLHDNARPHVAQPTLQKLNELGYEVLPHPPYSPDLLPTNYHVFKHLNNFLQGKRFHNQQDAENAFQEFVESQSTDFYATGINQLISRWQKCVDCNGSYFD

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 SET domain and mariner transposase fusion gene
别名 HsMar1, METNASE, SETMAR
计算分子量 671 aa, 77 kDa
观测分子量 77 kDa
GenBank蛋白编号BC008931
基因名称 SETMAR
Gene ID (NCBI) 6419
RRIDAB_2880248
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ53H47
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SETMAR antibody 25814-1-APDownload protocol
IP protocol for SETMAR antibody 25814-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Biol Chem

Structural and genome-wide analyses suggest that transposon-derived protein SETMAR alters transcription and splicing.

Authors - Qiujia Chen
View Article
humanWB

Cell Death Differ

A novel role of exostosin glycosyltransferase 2 (EXT2) in glioblastoma cell metabolism, radiosensitivity and ferroptosis

Authors - Rocío Matesanz-Sánchez
View Article