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SETMAR Polyclonal antibody

SETMAR Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 25814-1-AP

Print datasheet

Synonyms

HsMar1, METNASE, SETMAR

验证数据展示

  • WB analysis of HL-60 using 25814-1-AP

    WB analysis of HL-60 using 25814-1-AP

    HL-60 cells were subjected to SDS PAGE followed by western blot with 25814-1-AP (SETMAR Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IP experiment of HL-60 using 25814-1-AP

    IP experiment of HL-60 using 25814-1-AP

    IP result of anti-SETMAR (IP:25814-1-AP, 4ug; Detection:25814-1-AP 1:500) with HL-60 cells lysate 1600ug.

  • WB analysis using 25814-1-AP

    WB analysis using 25814-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25814-1-AP (SETMAR antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHL-60 cells, L02 cells, HEK-293T cells, Jurkat cells
Positive IP detected inHL-60 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

The immunogen of 25814-1-AP is SETMAR Fusion Protein expressed in E. coli.

Tested Applications IP, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen SETMAR fusion protein Ag22938 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name SET domain and mariner transposase fusion gene
Synonyms HsMar1, METNASE, SETMAR
Calculated Molecular Weight 671 aa, 77 kDa
Observed Molecular Weight 77 kDa
GenBank Accession NumberBC008931
Gene Symbol SETMAR
Gene ID (NCBI) 6419
RRIDAB_2880248
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ53H47
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SETMAR antibody 25814-1-APDownload protocol
IP protocol for SETMAR antibody 25814-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Biol Chem

Structural and genome-wide analyses suggest that transposon-derived protein SETMAR alters transcription and splicing.

Authors - Qiujia Chen
View Article