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验证数据展示

  • WB analysis using 68569-1-Ig (same clone as 68569-1-PBS)

    WB analysis using 68569-1-Ig (same clone as 68569-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68569-1-Ig (SETD7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68569-1-PBS in a different storage buffer formulation.

  • WB analysis using 68569-1-Ig (same clone as 68569-1-PBS)

    WB analysis using 68569-1-Ig (same clone as 68569-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68569-1-Ig (SETD7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68569-1-PBS in a different storage buffer formulation.

SETD7 Monoclonal antibody, PBS Only

SETD7 Monoclonal Antibody for WB, Indirect ELISA
Cat No. 68569-1-PBS

产品说明书

CloneNo. 1C1A9

宿主/亚型

Mouse / IgG1

种属反应性

Human, mouse, rat

应用

WB, Indirect ELISA

别名

FLJ21193, H3 K4 HMTase SETD7, KIAA1717, KMT7, Lysine N methyltransferase 7, SET7, SET7/9, SET9, SETD7

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

68569-1-PBS targets SETD7 in WB, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

经测试应用 WB, Indirect ELISA Application Description
经测试反应性 Human, mouse, rat
免疫原

CatNo: Ag33803

Product name: Recombinant human SETD7 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-366 aa of BC121055

Sequence: MDSDDEMVEEAVEGHLDDDGLPHGFCTVTYSSTDRFEGNFVHGEKNGRGKFFFFDGSTLEGYYVDDALQGQGVYTYEDGGVLQGTYVDGELNGPAQEYDTDGRLIFKGQYKDNIRHGVCWIYYPDGGSLVGEVNEDGEMTGEKIAYVYPDERTALYGKFIDGEMIEGKLATLMSTEEGRPHFELMPGNSVYHFDKSTSSCISTNALLPDPYESERVYVAESLISSAGEGLFSKVAVGPNTVMSFYNGVRITHQEVDSRDWALNGNTLSLDEETVIDVPEPYNHVSKYCASLGHKANHSFTPNCIYDMFVHPRFGPIKCIRTLRAVEADEELTVAYGYDHSPPGKSGPEAPEWYQVELKAFQATQQK

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 SET domain containing (lysine methyltransferase) 7
别名 FLJ21193, H3 K4 HMTase SETD7, KIAA1717, KMT7, Lysine N methyltransferase 7, SET7, SET7/9, SET9, SETD7
计算分子量 366 aa, 41 kDa
观测分子量48-50 kDa
GenBank蛋白编号BC121055
基因名称 SETD7
Gene ID (NCBI) 80854
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ8WTS6
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SET domain containing lysine methyltransferase 7 (SETD7, also called SET7/9) was the first lysine methyltransferase (KMT) discovered to specifically monomethylate lysine-4 of histone 3 (H3K4me1) (PMID: 26435321). SETD7 has been shown to have a very broad target specificity in vitro, including transcriptional regulators such as TAF10, p53, ER, p65, STAT3, Rb, Mypt, Tat, and Foxo3 (PMID: 25136132, 30013796).

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