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验证数据展示

  • Cytometric bead array sample test of MP51900-1

    Cytometric bead array sample test of MP51900-1

    Cytometric bead array sample test of MP51900-1,SETD7 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68569-1-PBS. Detection antibody: 68569-2-PBS.

  • WB analysis using 68569-1-Ig (same clone as 68569-1-PBS)

    WB analysis using 68569-1-Ig (same clone as 68569-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68569-1-Ig (SETD7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68569-1-PBS in a different storage buffer formulation.

  • WB analysis using 68569-1-Ig (same clone as 68569-1-PBS)

    WB analysis using 68569-1-Ig (same clone as 68569-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68569-1-Ig (SETD7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68569-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP51900-1

    Cytometric bead array standard curve of MP51900-1

    Cytometric bead array standard curve of MP51900-1, SETD7 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68569-1-PBS. Detection antibody: 68569-2-PBS. Standard:Ag33803. Range: 0.781-100 ng/mL.

SETD7 Monoclonal antibody, PBS Only (Capture)

SETD7 Monoclonal Antibody for WB, Cytometric bead array, Indirect ELISA
Cat No. 68569-1-PBS

产品说明书

CloneNo. 1C1A9

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, Cytometric bead array, Indirect ELISA

别名

1C1A9, EC:2.1.1.364, H3 K4 HMTase SETD7, H3-K4-HMTase SETD7, Histone H3-K4 methyltransferase SETD7

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

68569-1-PBS targets SETD7 as part of a matched antibody pair:

MP51900-1: 68569-1-PBS capture and 68569-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag33803

Product name: Recombinant human SETD7 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-366 aa of BC121055

Sequence: MDSDDEMVEEAVEGHLDDDGLPHGFCTVTYSSTDRFEGNFVHGEKNGRGKFFFFDGSTLEGYYVDDALQGQGVYTYEDGGVLQGTYVDGELNGPAQEYDTDGRLIFKGQYKDNIRHGVCWIYYPDGGSLVGEVNEDGEMTGEKIAYVYPDERTALYGKFIDGEMIEGKLATLMSTEEGRPHFELMPGNSVYHFDKSTSSCISTNALLPDPYESERVYVAESLISSAGEGLFSKVAVGPNTVMSFYNGVRITHQEVDSRDWALNGNTLSLDEETVIDVPEPYNHVSKYCASLGHKANHSFTPNCIYDMFVHPRFGPIKCIRTLRAVEADEELTVAYGYDHSPPGKSGPEAPEWYQVELKAFQATQQK

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 SET domain containing (lysine methyltransferase) 7
别名 1C1A9, EC:2.1.1.364, H3 K4 HMTase SETD7, H3-K4-HMTase SETD7, Histone H3-K4 methyltransferase SETD7
计算分子量 366 aa, 41 kDa
观测分子量48-50 kDa
GenBank蛋白编号BC121055
基因名称 SETD7
Gene ID (NCBI) 80854
RRIDAB_3085271
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ8WTS6
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SET domain containing lysine methyltransferase 7 (SETD7, also called SET7/9) was the first lysine methyltransferase (KMT) discovered to specifically monomethylate lysine-4 of histone 3 (H3K4me1) (PMID: 26435321). SETD7 has been shown to have a very broad target specificity in vitro, including transcriptional regulators such as TAF10, p53, ER, p65, STAT3, Rb, Mypt, Tat, and Foxo3 (PMID: 25136132, 30013796).

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