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Species Reactivity
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Conjugate
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验证数据展示

  • WB analysis of RAW 264.7 using 24377-1-AP

    WB analysis of RAW 264.7 using 24377-1-AP

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 24377-1-AP (SETD6 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

SETD6 Polyclonal antibody

SETD6 Polyclonal Antibody for WB, ELISA
Cat No. 24377-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, ELISA

别名

SET domain containing 6, SETD6

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inRAW 264.7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24377-1-AP targets SETD6 in WB, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag19485

Product name: Recombinant human SETD6 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 124-473 aa of BC022451

Sequence: PLLLALLHELQAPASRWRPYFALWPELGRLEHPMFWPEEERRCLLQGTGVPEAVEKDLANISSEYQSIVLPFMEAHPDLFSLGVRSLELYHQLVALVMAYSFQEPLEEEEDEKEPNSPVMVPAADILNHLANHNANLEYSANCLRMVATQPIPKGHEIFNTYGQMANWQLIHMYGFVEPYPDNTDDTADIQMVTVREAALQGTKTEAERHLVYERWDFLCKLEMVGEEGAFVIGREEVLTEEELTTTLKVLCMPAEEFRELKDQDGGGDDKREEGSLTITNIPKLKASWRQLLQNSVLLTLQTYATDLKTDQGLLSNKEVYAKLSWREQQALQVRYGQKMILHQLLELTS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 SET domain containing 6
别名 SET domain containing 6, SETD6
计算分子量 473 aa, 53 kDa
观测分子量 53 kDa
GenBank蛋白编号BC022451
基因名称 SETD6
Gene ID (NCBI) 79918
RRIDAB_2879514
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8TBK2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SETD6 also named as SET domain containing 6 is a 473 amino-acid protein, which localizes in the Nucleus. The molecular weight of SETD6 is 53kDa. SETD6 consists of 1 SET domain and belongs to the class V-like SAM-binding methyltransferase superfamily. SETD6 as a protein-lysine N-methyltransferase that specifically monomethylates at Lysine 310 of the RELA subunit of NF-kappa-B complex, leading to down-regulate NF-kappa-B transcription factor activity.

实验方案

Product Specific Protocols
WB protocol for SETD6 antibody 24377-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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