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验证数据展示

  • IHC staining of human placenta using 84622-1-RR

    IHC staining of human placenta using 84622-1-RR

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84622-1-RR (SETD5 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of U2OS using 84622-1-RR

    IF Staining of U2OS using 84622-1-RR

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using SETD5 antibody (84622-1-RR, Clone: 241903A3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 84622-1-RR

    Affinity and Kinetic Characterization of 84622-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84622-1-RR against Human SETD5 were performed. The affinity constant is 5.76 nM.

SETD5 Recombinant antibody

SETD5 Uni-rAbTM Recombinant Antibody for IHC, IF/ICC, ELISA
Cat No. 84622-1-RR

产品说明书

CloneNo. 241903A3

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, IF/ICC, ELISA

别名

241903A3, Histone-lysine N-methyltransferase SETD5, KIAA1757

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84622-1-RR targets SETD5 in IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 SETD5 fusion protein Ag28929 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 SET domain containing 5
别名 241903A3, Histone-lysine N-methyltransferase SETD5, KIAA1757
GenBank蛋白编号BC020956
基因名称 SETD5
Gene ID (NCBI) 55209
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9C0A6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SETD5, also known as KIAA1757, is a member of the histone lysine methyltransferase family. It has 3 isoforms and is involved in a variety of cellular functions, including cell cycle regulation and cell proliferation, and has a potential role in early embryonic development (PMID: 36875494).

实验方案

Product Specific Protocols
IHC protocol for SETD5 antibody 84622-1-RRDownload protocol
IF protocol for SETD5 antibody 84622-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols