验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, MG-63 cells, U2OS cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
30855-1-AP targets SETD4 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag33842 Product name: Recombinant human SETD4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-50 aa of BC002898 Sequence: MQKGKGRTSRIRRRKLCGSSESRGVNESHKSEFIELRKWLKARKFQDSNL 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | SET domain containing 4 |
| 别名 | C21orf18, C21orf27, SET domain containing 4, SETD 4 |
| 计算分子量 | 440 aa, 50 kDa |
| 观测分子量 | 40 kDa |
| GenBank蛋白编号 | BC002898 |
| 基因名称 | SETD4 |
| Gene ID (NCBI) | 54093 |
| RRID | AB_3742378 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | Q9NVD3 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
As a lysine methyl-transferase of histone, SET domain-containing protein 4 (SETD4) has been reported to play a role in cell proliferation by controlling related gene expressions . Limited reports reveal that SETD4 is not only indispensable for embryonic neurogenesis, development and regeneration of pancreatic and intestinal organs, regulation of bone marrow mesenchymal stem cells, and modulation of inflammatory cytokines of macrophages, but also involved in the tumorigenesis, progression, and chemotherapy resistance of various tumors, including liver cancer, breast cancer, lymphoma and Non-small Cell Lung Cancer. (PMID: 35545041, PMID: 36027907, PMID: 37879429)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SETD4 antibody 30855-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
