验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, U2OS cells, K-562 cells |
| Positive IHC detected in | human colon cancer tissue, human oesophagus cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
55377-1-AP targets SETD2 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC, CoIP |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | SET domain containing 2 |
| 别名 | HIF 1, HIF1, HIP 1, hSET2, HSPC069, Huntingtin yeast partner B, HYPB, KIAA1732, KMT3A, Lysine N methyltransferase 3A, p231HBP, SET domain containing 2, SET2, SETD2 |
| 计算分子量 | 288 kDa |
| 观测分子量 | 287 kDa |
| GenBank蛋白编号 | NM_014159 |
| 基因名称 | SETD2 |
| Gene ID (NCBI) | 29072 |
| RRID | AB_2881318 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9BYW2 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SETD2, also named as HIF1, HYPB, KIAA1732, KMT3A, SET2, p231HBP, hSET2 and HIP-1, belongs to the histone-lysine methyltransferase family and SET2 subfamily. It is a histone methyltransferase that methylates 'Lys-36' of histone H3. SETD2 probably plays a role in chromatin structure modulation during elongation via its interaction with hyperphosphorylated POLR2A. It binds DNA at promoters. SETD2 may also act as a transcription activator that binds to promoters.(PMID:16118227) It is a potential tumor suppressor gene in breast cancer.(PMID:20944102) This antibody is specific to SETD2.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SETD2 antibody 55377-1-AP | Download protocol |
| IHC protocol for SETD2 antibody 55377-1-AP | Download protocol |
| IF protocol for SETD2 antibody 55377-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nucleic Acids Res PHD finger protein 1 (PHF1) is a novel reader for histone H4R3 symmetric dimethylation and coordinates with PRMT5-WDR77/CRL4B complex to promote tumorigenesis.
| ||
Cell Death Dis Knockdown of SETD2 promotes erastin-induced ferroptosis in ccRCC
| ||
Carcinogenesis Proteomic analysis of the OGT interactome: novel links to epithelial-mesenchymal transition and metastasis of cervical cancer. | ||
J Exp Clin Cancer Res A novel FBW7/NFAT1 axis regulates cancer immunity in sunitinib-resistant renal cancer by inducing PD-L1 expression. | ||
Function (Oxf) Structure and Function of RhoBTB1 Required for Substrate Specificity and Cullin-3 Ubiquitination |
