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验证数据展示

  • WB analysis of human brain using 55005-1-AP (same clone as 55005-1-PBS)

    WB analysis of human brain using 55005-1-AP (same clone as 55005-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 55005-1-AP (SETD1B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55005-1-PBS in a different storage buffer formulation.

  • IP experiment of MCF-7 using 55005-1-AP (same clone as 55005-1-PBS)

    IP experiment of MCF-7 using 55005-1-AP (same clone as 55005-1-PBS)

    IP result of anti-SETD1B (IP:55005-1-AP, 5ug; Detection:55005-1-AP 1:300) with MCF-7 cells lysate 3250ug. This data was developed using the same antibody clone with 55005-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 55005-1-AP (same clone as 55005-1-PBS)

    IHC staining of human breast cancer using 55005-1-AP (same clone as 55005-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 55005-1-AP (SETD1B Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 55005-1-PBS in a different storage buffer formulation.

  • IHC staining of human ovary tumor using 55005-1-AP (same clone as 55005-1-PBS)

    IHC staining of human ovary tumor using 55005-1-AP (same clone as 55005-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 55005-1-AP (SETD1B Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 55005-1-PBS in a different storage buffer formulation.

  • IHC staining of human ovary tumor using 55005-1-AP (same clone as 55005-1-PBS)

    IHC staining of human ovary tumor using 55005-1-AP (same clone as 55005-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 55005-1-AP (SETD1B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55005-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 55005-1-AP (same clone as 55005-1-PBS)

    IF Staining of HepG2 using 55005-1-AP (same clone as 55005-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SETD1B antibody (55005-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 55005-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

SETD1B Polyclonal antibody, PBS Only

SETD1B Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 55005-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

EC:2.1.1.364, Histone-lysine N-methyltransferase SETD1B, hSET1B, KIAA1076, KMT2G

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

55005-1-PBS targets SETD1B in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 SET domain containing 1B
别名 EC:2.1.1.364, Histone-lysine N-methyltransferase SETD1B, hSET1B, KIAA1076, KMT2G
计算分子量 209 kDa
观测分子量 250-260 kDa, 450 kDa
GenBank蛋白编号NM_015048
基因名称 SETD1B
Gene ID (NCBI) 23067
RRIDAB_10858389
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UPS6
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SETD1B, also named as KMT2G. Set1B associates with a about 450 kDa complex that contains all five non-catalytic components of the Set1A complex, including CFP1, Rbbp5, Ash2, Wdr5, and Wdr82. The complex is a histone methyltransferase that produces trimethylated histone H3 at Lys4. Set1B is widely expressed, and localize to a largely non-overlapping set of euchromatic nuclear speckles, suggesting that Set1B bind to a unique set of target genes and thus make non-redundant contributions to the epigenetic control of chromatin structure and gene expression( PMID: 17355966). The antibody is specific to SETD1B and, in westernbloting experiments, two specific bands can be detected, about 250 kDa, 450 kDa respectively, in addition, a unknown band around 70 kDa. This antibody is a rabbit polyclonal antibody raised against a region of human SETD1B.

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