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验证数据展示

  • WB analysis using 23911-1-AP

    WB analysis using 23911-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 23911-1-AP (SOLO antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • WB analysis using 23911-1-AP

    WB analysis using 23911-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 23911-1-AP (SOLO/SESTD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse brain using 23911-1-AP

    IP experiment of mouse brain using 23911-1-AP

    IP result of anti-SOLO (IP:23911-1-AP, 4ug; Detection:23911-1-AP 1:4000) with mouse brain tissue lysate 1280 ug.

  • IF Staining of HepG2 using 23911-1-AP

    IF Staining of HepG2 using 23911-1-AP

    Immunofluorescent analysis of HepG2 cells using 23911-1-AP (SOLO antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IP experiment of rat brain using 23911-1-AP

    IP experiment of rat brain using 23911-1-AP

    IP result of anti-SOLO (IP:23911-1-AP, 0ug; Detection:23911-1-AP 1:4000) with rat brain tissue lysate 1120 ug.

  • IF Staining of Jurkat using 23911-1-AP

    IF Staining of Jurkat using 23911-1-AP

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using SOLO antibody (23911-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

SOLO/SESTD1 Polyclonal antibody

SOLO/SESTD1 Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Cat No. 23911-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, IP, ELISA

别名

SESTD1, SOLO, Huntingtin-interacting protein-like protein, Protein Solo, SEC14 and spectrin domains 1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHepG2 cells, Jurkat cells, MCF-7 cells, mouse brain tissue, rat brain tissue, L02 cells
Positive IP detected inmouse brain tissue, rat brain tissue
Positive IF/ICC detected inJurkat cells, HepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

23911-1-AP targets SOLO/SESTD1 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag21016

Product name: Recombinant human SESTD1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 348-696 aa of BC047578

Sequence: DLVELKSLQQQLSDVCYRQASQLEFRQNLLQAALEFHGVAQDLSQQLDGLLGMLCVDVAPADGASIQQTLKLLEEKLKSVDVGLQGLREKGQGLLDQISNQASWAYGKDVTIENKENVDHIQGVMEDMQLRKQRCEDMVDVRRLKMLQMVQLFKCEEDAAQAVEWLSELLDALLKTHIRLGDDAQETKVLLEKHRKFVDVAQSTYDYGRQLLQATVVLCQSLRCTSRSSGDTLPRLNRVWKQFTIASEERVHRLEMAIAFHSNAEKILQDCPEEPEAINDEEQFDEIEAVGKSLLDRLTVPVVYPDGTEQYFGSPSDMASTAENIRDRMKLVNLKRQQLRHPEMVTTES

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 SEC14 and spectrin domains 1
别名 SESTD1, SOLO, Huntingtin-interacting protein-like protein, Protein Solo, SEC14 and spectrin domains 1
计算分子量 696 aa, 79 kDa
观测分子量 70-79 kDa
GenBank蛋白编号BC047578
基因名称 SOLO
Gene ID (NCBI) 91404
RRIDAB_2879360
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen Affinity purified
UNIPROT IDQ86VW0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SEC14 domain and spectrin repeat-containing protein 1 (SESTD1) is also named Huntingtin-interacting protein-like protein and SOLO. SESTD1, a previously uncharacterized lipid-binding protein strongly expressed in aorta, was found to interact with both TRPC proteins and modulate receptor-activated TRPC5 currents in a recombinant cell line (PMID: 20164195). SESTD1 is proposed to be involved in phospholipid regulation of transient receptor potential channels TRPC4 and TRPC5 (PMID: 26503763). SESTD1 expression is associated with high abundance of immune infiltration, including T helper cells, Th2 cells, and Macrophages in HCC (PMID: 41016345).

实验方案

Product Specific Protocols
IF protocol for SOLO/SESTD1 antibody 23911-1-APDownload protocol
IP protocol for SOLO/SESTD1 antibody 23911-1-APDownload protocol
WB protocol for SOLO/SESTD1 antibody 23911-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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