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SOLO Polyclonal antibody

SOLO Polyclonal Antibody for IF/ICC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 23911-1-AP

Print datasheet

Synonyms

Protein Solo, SEC14 and spectrin domains 1, SESTD1, SOLO

验证数据展示

  • WB analysis using 23911-1-AP

    WB analysis using 23911-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 23911-1-AP (SOLO antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse brain using 23911-1-AP

    IP experiment of mouse brain using 23911-1-AP

    IP result of anti-SOLO (IP:23911-1-AP, 4ug; Detection:23911-1-AP 1:4000) with mouse brain tissue lysate 1280 ug.

  • IF Staining of HepG2 using 23911-1-AP

    IF Staining of HepG2 using 23911-1-AP

    Immunofluorescent analysis of HepG2 cells using 23911-1-AP (SOLO antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IP experiment of rat brain using 23911-1-AP

    IP experiment of rat brain using 23911-1-AP

    IP result of anti-SOLO (IP:23911-1-AP, 0ug; Detection:23911-1-AP 1:4000) with rat brain tissue lysate 1120 ug.

经过测试的应用

Positive WB detected inHepG2 cells, Jurkat cells, MCF-7 cells, mouse brain tissue, rat brain tissue
Positive IP detected inmouse brain tissue, rat brain tissue
Positive IF detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

23911-1-AP targets SOLO in WB, IP, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, IP, WB, ELISA
Tested Reactivity human, mouse, rat
Immunogen SOLO fusion protein Ag21016 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name SEC14 and spectrin domains 1
Synonyms Protein Solo, SEC14 and spectrin domains 1, SESTD1, SOLO
Calculated Molecular Weight 696 aa, 79 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC047578
Gene Symbol SESTD1
Gene ID (NCBI) 91404
RRIDAB_2879360
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
UNIPROT IDQ86VW0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SOLO antibody 23911-1-APDownload protocol
IF protocol for SOLO antibody 23911-1-APDownload protocol
IP protocol for SOLO antibody 23911-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols