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验证数据展示

  • WB analysis of mouse brain using 55010-1-AP

    WB analysis of mouse brain using 55010-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 55010-1-AP (SESN1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse brain using 55010-1-AP

    IP experiment of mouse brain using 55010-1-AP

    IP result of anti-SESN1 (IP:55010-1-AP, 4ug; Detection:55010-1-AP 1:500) with mouse brain tissue lysate 3440ug.

SESN1 Polyclonal antibody

SESN1 Polyclonal Antibody for WB, IP, ELISA
Cat No. 55010-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (1)

应用

WB, IP, ELISA

别名

p53 regulated protein PA26, PA26, RP11 787I22.1, SESN1, SEST1, sestrin 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue
Positive IP detected inmouse brain tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

55010-1-AP targets SESN1 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, ELISA Application Description
文献引用应用WB, IP
经测试反应性 human, mouse, rat
文献引用反应性pig
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 sestrin 1
别名 p53 regulated protein PA26, PA26, RP11 787I22.1, SESN1, SEST1, sestrin 1
计算分子量 64 kDa
观测分子量 50-57 kDa
GenBank蛋白编号NM_014454
基因名称 SESN1
Gene ID (NCBI) 27244
RRIDAB_10858791
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y6P5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

Sestrins, including sestrin-1 (PA26), sestrin-2 (Hi95), and sestrin-3, are 48 to 65 kDa cystein sulfinyl reductases and they modulate peroxide signaling and antioxidant defense. These proteins selectively reduce or repair hyperoxidized forms of typical 2-Cys peroxiredoxins within eukaryotes. Expression of these proteins is regulated by p53, a tumor suppressor protein. Sestrin 1 is implicated in the inhibition of cell growth. It is approximately a 66kDa protein in humans (551 amino acids). This antibody is specific to Sestrin 1.

实验方案

Product Specific Protocols
WB protocol for SESN1 antibody 55010-1-APDownload protocol
IP protocol for SESN1 antibody 55010-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
pigWB, IP

J Nutr

Intermittent Bolus Compared With Continuous Feeding Enhances Insulin and Amino Acid Signaling to Translation Initiation in Skeletal Muscle of Neonatal Pigs.

Authors - Agus Suryawan
View Article