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SESN1 Polyclonal antibody

SESN1 Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 55010-1-AP

Print datasheet

Synonyms

p53 regulated protein PA26, PA26, RP11 787I22.1, SESN1, SEST1, sestrin 1

验证数据展示

  • WB analysis of mouse brain using 55010-1-AP

    WB analysis of mouse brain using 55010-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 55010-1-AP (SESN1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse brain using 55010-1-AP

    IP experiment of mouse brain using 55010-1-AP

    IP result of anti-SESN1 (IP:55010-1-AP, 4ug; Detection:55010-1-AP 1:500) with mouse brain tissue lysate 3440ug.

经过测试的应用

Positive WB detected inmouse brain tissue
Positive IP detected inmouse brain tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

55010-1-AP targets SESN1 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IP, WB, ELISA
Cited ApplicationsWB, IP
Tested Reactivity human, mouse, rat
Cited Reactivitypig
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name sestrin 1
Synonyms p53 regulated protein PA26, PA26, RP11 787I22.1, SESN1, SEST1, sestrin 1
Calculated Molecular Weight 64 kDa
Observed Molecular Weight 50-57 kDa
GenBank Accession NumberNM_014454
Gene Symbol SESN1
Gene ID (NCBI) 27244
RRIDAB_10858791
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y6P5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

Sestrins, including sestrin-1 (PA26), sestrin-2 (Hi95), and sestrin-3, are 48 to 65 kDa cystein sulfinyl reductases and they modulate peroxide signaling and antioxidant defense. These proteins selectively reduce or repair hyperoxidized forms of typical 2-Cys peroxiredoxins within eukaryotes. Expression of these proteins is regulated by p53, a tumor suppressor protein. Sestrin 1 is implicated in the inhibition of cell growth. It is approximately a 66kDa protein in humans (551 amino acids). This antibody is specific to Sestrin 1.

实验方案

Product Specific Protocols
WB protocol for SESN1 antibody 55010-1-APDownload protocol
IP protocol for SESN1 antibody 55010-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
pigWB, IP

J Nutr

Intermittent Bolus Compared With Continuous Feeding Enhances Insulin and Amino Acid Signaling to Translation Initiation in Skeletal Muscle of Neonatal Pigs.

Authors - Agus Suryawan
View Article