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验证数据展示

  • WB analysis of mouse pancreas using 13298-1-AP

    WB analysis of mouse pancreas using 13298-1-AP

    mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 13298-1-AP (SERPINI2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse pancreas using 13298-1-AP

    IHC staining of mouse pancreas using 13298-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 13298-1-AP (SERPINI2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse pancreas using 13298-1-AP

    IHC staining of mouse pancreas using 13298-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 13298-1-AP (SERPINI2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

SERPINI2 Polyclonal antibody

SERPINI2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 13298-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

MEPI, PANCPIN, Peptidase inhibitor 14, PI 14, PI14, Serpin I2, SERPINI2, TSA2004

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse pancreas tissue
Positive IHC detected inmouse pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13298-1-AP targets SERPINI2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag4022

Product name: Recombinant human SERPINI2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 66-415 aa of BC027859

Sequence: LEMVQLGAKGKAQQQIRQTLKQQETSAGEEFFVLKSFFSAISEKKQEFTFNLANALYLQEGFTVKEQYLHGNKEFFQSAIKLVDFQDAKACAEMISTWVERKTDGKIKDMFSGEEFGPLTRLVLVNAIYFKGDWKQKFRKEDTQLINFTKKNGSTVKIPMMKALLRTKYGYFSESSLNYQVLELSYKGDEFSLIIILPAEGMDIEEVEKLITAQQILKWLSEMQEEEVEISLPRFKVEQKVDFKDVLYSLNITEIFSGGCDLSGITDSSEVYVSQVTQKVFFEINEDGSEAATSTGIHIPVIMSLAQSQFIANHPFLFIMKHNPTESILFMGRVTNPDTQEIKGRDLDSL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 serpin peptidase inhibitor, clade I (pancpin), member 2
别名 MEPI, PANCPIN, Peptidase inhibitor 14, PI 14, PI14, Serpin I2, SERPINI2, TSA2004
计算分子量 405 aa, 46 kDa
观测分子量 46 kDa
GenBank蛋白编号BC027859
基因名称 SERPINI2
Gene ID (NCBI) 5276
RRIDAB_2185341
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO75830
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SERPINI2 antibody 13298-1-APDownload protocol
IHC protocol for SERPINI2 antibody 13298-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols