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验证数据展示

  • WB analysis using 21719-1-AP

    WB analysis using 21719-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21719-1-AP (SERPINB10 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 21719-1-AP

    IF Staining of HepG2 using 21719-1-AP

    Immunofluorescent analysis of HepG2 cells, using SERPINB10 antibody 21719-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • IHC staining of human tonsillitis using 21719-1-AP

    IHC staining of human tonsillitis using 21719-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 21719-1-AP (SERPINB10 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Featured Product
  • KD/KO验证

SERPINB10 Polyclonal antibody

SERPINB10 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 21719-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

bomapin, Peptidase inhibitor 10, PI 10, PI10, Serpin B10, SERPINB10

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inA431 cells, HL-60 cells, Sp2/0 cells, human saliva
Positive IHC detected inhuman tonsillitis tissue, human pancreas tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21719-1-AP targets SERPINB10 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原

CatNo: Ag16372

Product name: Recombinant human SERPINB10 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-307 aa of BC096217

Sequence: MDSLATSINQFALELSKKLAESAQGKNIFFSSWSISTSLTIVYLGAKGTTAAQMAQVLQFNRDQGVKCDPESEKKRKMEFNLSNSEEIHSDFQTLISEILKPNDDYLLKTANAIYGEKTYAFHNKYLEDMKTYFGAEPQPVNFVEASDQIRKDINSWVERQTEGKIQNLLPDDSVDSTTRMILVNALYFKGIWEHQFLVQNTTEKPFRINETTSKPVQMMFMKKKLHIFHIEKPKAVGLQLYYKSRDLSLLILLPEDINGLEQLEKAITYEKLNEWTSADMMELYEVQLHLPKFKLEDSYDLKSTLS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 serpin peptidase inhibitor, clade B (ovalbumin), member 10
别名 bomapin, Peptidase inhibitor 10, PI 10, PI10, Serpin B10, SERPINB10
计算分子量 397 aa, 45 kDa
观测分子量 45 kDa
GenBank蛋白编号BC096217
基因名称 SERPINB10
Gene ID (NCBI) 5273
RRIDAB_10734592
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP48595
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SERPINB10 antibody 21719-1-APDownload protocol
IHC protocol for SERPINB10 antibody 21719-1-APDownload protocol
IF protocol for SERPINB10 antibody 21719-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,mouseWB,IHC

Am J Physiol Lung Cell Mol Physiol

Epithelial SERPINB10, a novel marker of airway eosinophilia in asthma, contributes to allergic airway inflammation.

Authors - Yuqing Mo
  • KD Validated
View Article