• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis of human plasma using 66454-1-Ig

    WB analysis of human plasma using 66454-1-Ig

    human plasma were subjected to SDS PAGE followed by western blot with 66454-1-Ig (SERPINA7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 66454-1-Ig

    IHC staining of human liver cancer using 66454-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66454-1-Ig (SERPINA7 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 66454-1-Ig

    IHC staining of human liver cancer using 66454-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66454-1-Ig (SERPINA7 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of human liver cancer using 66454-1-Ig

    IF Staining of human liver cancer using 66454-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using SERPINA7 antibody (66454-1-Ig, Clone: 1B11B10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • IF Staining of human liver cancer using 66454-1-Ig

    IF Staining of human liver cancer using 66454-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using SERPINA7 antibody (66454-1-Ig, Clone: 1B11B10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

SERPINA7 Monoclonal antibody

SERPINA7 Monoclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 66454-1-Ig

产品说明书

CloneNo. 1B11B10
引用文献(1)

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, pig, rat

应用

WB, IHC, IF-P, ELISA

别名

Serpin A7, SERPINA7, T4 binding globulin, TBG, Thyroxine binding globulin

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite®594
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inhuman plasma tissue, human testis tissue
Positive IHC detected inhuman liver cancer tissue, mouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman liver cancer tissue
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 1 publications below

产品信息

66454-1-Ig targets SERPINA7 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, pig, rat samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
文献引用应用IHC
经测试反应性 human, mouse, pig, rat
文献引用反应性human
免疫原 SERPINA7 fusion protein Ag9823 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 7
别名 Serpin A7, SERPINA7, T4 binding globulin, TBG, Thyroxine binding globulin
计算分子量 415 aa, 46 kDa
观测分子量 50 kDa
GenBank蛋白编号BC020747
基因名称 SERPINA7
Gene ID (NCBI) 6906
RRIDAB_2881823
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP05543
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SERPINA7 antibody 66454-1-IgDownload protocol
IHC protocol for SERPINA7 antibody 66454-1-IgDownload protocol
IF protocol for SERPINA7 antibody 66454-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Heliyon

Secreted proteins in plasma and placenta as novel non-invasive biomarkers for intrahepatic cholestasis of pregnancy: A case-control study

Authors - Lingyan Chen
View Article