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验证数据展示

  • WB analysis of HepG2 using 17451-1-AP

    WB analysis of HepG2 using 17451-1-AP

    WB result of Serpin A12/Vaspin antibody (17451-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-Serpin A12/Vaspin transfected HepG2 cells.

  • WB analysis of human adipose using 17451-1-AP

    WB analysis of human adipose using 17451-1-AP

    human adipose tissue were subjected to SDS PAGE followed by western blot with 17451-1-AP (SERPINA12 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Serpin A12/Vaspin Polyclonal antibody

Serpin A12/Vaspin Polyclonal Antibody for WB, ELISA
Cat No. 17451-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human and More (2)

应用

WB, ELISA

别名

SERPINA12, SERPINA12/Vaspin, Vaspin, OL 64, OL-64

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inhuman adipose tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

17451-1-AP targets Serpin A12/Vaspin in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性mouse, rat
免疫原

CatNo: Ag11574

Product name: Recombinant human SERPINA12 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-414 aa of BC040857

Sequence: MNPTLGLAIFLAVLLTVKGLLKPSFSPRNYKALSEVQGWKQRMAAKELARQNMDLGFKLLKKLAFYNPGRNIFLSPLSISTAFSMLCLGAQDSTLDEIKQGFNFRKMPEKDLHEGFHYIIHELTQKTQDLKLSIGNTLFIDQRLQPQRKFLEDAKNFYSAETILTNFQNLEMAQKQINDFISQKTHGKINNLIENIDPGTVMLLANYIFFRARWKHEFDPNVTKEEDFFLEKNSSVKVPMMFRSGIYQVGYDDKLSCTILEIPYQKNITAIFILPDEGKLKHLEKGLQVDTFSRWKTLLSRRVVDVSVPRLHMTGTFDLKKTLSYIGVSKIFEEHGDLTKIAPHRSLKVGEAVHKAELKMDERGTEGAAGTGAQTLPMETPLVVKIDKPYLLLIYSEKIPSVLFLGKIVNPIGK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 12
别名 SERPINA12, SERPINA12/Vaspin, Vaspin, OL 64, OL-64
计算分子量 414 aa, 47 kDa
观测分子量 47-50 kDa
GenBank蛋白编号BC040857
基因名称 Vaspin
Gene ID (NCBI) 145264
RRIDAB_2878408
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8IW75
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SERPINA12 is also named as Serpin A12 and Visceral adipose tissue-derived serine protease inhibitor (Vaspin). Vaspin is an adipokine belonging to the serpin family, thus also named SERPINA12, with targets in kallikrein 7 and 14 that participates in skin desquamation (PMID: 34359881). Vaspin is a compensatory molecule in obesity and insulin resistance (PMID: 18321232). Vaspin is a newly described adipokine with an insulin sensitizing effect and inhibitory impact on food intake (PMID: 16030142, PMID: 21465327).

实验方案

Product Specific Protocols
WB protocol for Serpin A12/Vaspin antibody 17451-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
rat,mouseWB

Biochem Biophys Res Commun

Vaspin alleviates myocardial ischaemia/reperfusion injury via activating autophagic flux and restoring lysosomal function.

Authors - Feihong Yang
View Article