SERPINA10 Monoclonal antibody
SERPINA10 Monoclonal Antibody for IF/ICC,IF-P, IHC, WB, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
human, rat, pig
Applications
IF/ICC,IF-P, IHC, WB, ELISA
Conjugate
Unconjugated
CloneNo.
5B3C9
验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, L02 cells, pig liver tissue, rat liver tissue |
| Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HepG2 cells, human liver tissue |
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1500-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IHC | See 1 publications below |
产品信息
60113-1-Ig targets SERPINA10 in WB, IF, IHC, ELISA applications and shows reactivity with human, rat, pig samples.
| Tested Applications | IF/ICC,IF-P, IHC, WB, ELISA |
| Cited Applications | IHC |
| Tested Reactivity | human, rat, pig |
| Cited Reactivity | human |
| Immunogen | SERPINA10 fusion protein Ag2420 种属同源性预测 |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 10 |
| Synonyms | PZI, Serpin A10, SERPINA10, ZPI |
| Calculated Molecular Weight | 444 aa, 51 kDa |
| Observed Molecular Weight | 51 kDa |
| GenBank Accession Number | BC022261 |
| Gene Symbol | SERPINA10 |
| Gene ID (NCBI) | 51156 |
| RRID | AB_2301526 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9UK55 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SERPINA10 belongs to the serpin family. It is predominantly expressed in the liver and secreted in plasma. It inhibits the activity of coagulation factors Xa and XIa in the presence of protein Z, calcium and phospholipid. Mutations in this gene are associated with venous thrombosis.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SERPINA10 antibody 60113-1-Ig | Download protocol |
| IHC protocol for SERPINA10 antibody 60113-1-Ig | Download protocol |
| IF protocol for SERPINA10 antibody 60113-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Front Oncol High Serpin Family A Member 10 Expression Confers Platinum Sensitivity and Is Associated With Survival Benefit in High-Grade Serous Ovarian Cancer: Based on Quantitative Proteomic Analysis. |
