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验证数据展示

  • WB analysis using 16382-1-AP

    WB analysis using 16382-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16382-1-AP (Alpha-1-Antitrypsin antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

  • WB analysis using 16382-1-AP

    WB analysis using 16382-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16382-1-AP (Alpha-1-Antitrypsin antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 16382-1-AP

    IHC staining of human liver cancer using 16382-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16382-1-AP (Alpha 1 Antitrypsin antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SERPINA1/AAT antibody included
  • IHC staining of mouse liver using 16382-1-AP

    IHC staining of mouse liver using 16382-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 16382-1-AP (Alpha 1 Antitrypsin antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SERPINA1/AAT antibody included
  • WB analysis of HepG2 using 16382-1-AP

    WB analysis of HepG2 using 16382-1-AP

    WB result of Alpha 1 Antitrypsin antibody (16382-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Alpha 1 Antitrypsin transfected HepG2 cells.

  • IHC staining of rat kidney using 16382-1-AP

    IHC staining of rat kidney using 16382-1-AP

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 16382-1-AP (Alpha 1 Antitrypsin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SERPINA1/AAT antibody included
  • IHC staining of rat kidney using 16382-1-AP

    IHC staining of rat kidney using 16382-1-AP

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 16382-1-AP (Alpha 1 Antitrypsin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SERPINA1/AAT antibody included
  • IHC staining of mouse kidney using 16382-1-AP

    IHC staining of mouse kidney using 16382-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 16382-1-AP (Alpha 1 Antitrypsin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SERPINA1/AAT antibody included
  • FC experiment of HepG2 using 16382-1-AP

    FC experiment of HepG2 using 16382-1-AP

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human Alpha 1 Antitrypsin (16382-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of HepG2 using 16382-1-AP

    IF Staining of HepG2 using 16382-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using Alpha 1 Antitrypsin antibody (16382-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IP experiment of mouse kidney using 16382-1-AP

    IP experiment of mouse kidney using 16382-1-AP

    IP result of anti-Alpha 1 Antitrypsin (IP:16382-1-AP, 4ug; Detection:16382-1-AP 1:5000) with mouse kidney tissue lysate 1600 ug.

  • IHC staining of human colon using 16382-1-AP

    IHC staining of human colon using 16382-1-AP

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 16382-1-AP (Alpha 1 Antitrypsin antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SERPINA1/AAT antibody included
  • IHC staining of human colon using 16382-1-AP

    IHC staining of human colon using 16382-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 16382-1-AP (Alpha 1 Antitrypsin antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SERPINA1/AAT antibody included
  • IF Staining of mouse liver using 16382-1-AP

    IF Staining of mouse liver using 16382-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using Alpha 1 Antitrypsin antibody (16382-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Featured Product
  • KD/KO验证

Alpha-1-Antitrypsin Polyclonal antibody

Alpha-1-Antitrypsin Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA
Cat No. 16382-1-AP

产品说明书

引用文献(29)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA and More (1)

别名

a1-antitrypsin, Serpin A1, SERPINA1, A1A, A-1-Antitrypsin

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, human kidney tissue, human placenta tissue, human urine sample, mouse liver tissue, mouse lung tissue, mouse skin tissue, HepG2 cells, mouse kidney tissue, human saliva
Positive IP detected inmouse kidney tissue
Positive IHC detected inmouse liver tissue, human colon tissue, human liver cancer tissue, mouse kidney tissue, rat kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse liver tissue
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy SERPINA1/AAT Ready-To-Use IHC Kit. SERPINA1/AAT primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16382-1-AP targets Alpha-1-Antitrypsin in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA Application Description
文献引用应用WB, IHC, IF, IP
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat
免疫原 Alpha-1-Antitrypsin fusion protein Ag9369 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1
别名 a1-antitrypsin, Serpin A1, SERPINA1, A1A, A-1-Antitrypsin
计算分子量 418 aa, 47 kDa
观测分子量 47-51 kDa
GenBank蛋白编号BC015642
基因名称 Alpha 1-Antitrypsin
Gene ID (NCBI) 5265
RRIDAB_10641185
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP01009
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SERPINA1 is the gene for a protein called alpha-1-antitrypsin (AAT), which is a serine protease inhibitor whose targets include elastase, plasmin, thrombin, trypsin, chymotrypsin, and plasminogen activator. AAT is a glycoprotein synthesized primarily by hepatocytes, with smaller amountssynthesized by intestinal epithelial cells, neutrophils, pulmonary alveolar cells and macrophages. AAT is the most abundant, endogenous serine protease inhibitor in blood circulation and it has been implicated in regulating vital fluid phase biological events such as blood coagulation, fibrinolysis, complement activation, apoptosis, reproduction, tumor progression and inflammatory response. The primary function of AAT is thought to be the inactivation of neutrophil elastase and other endogenous serine proteases. Defects in SERPINA1 can cause emphysema or liver disease. Alpha 1 Antitrypsin has two bands (PMID: 36703990, PMID: 26869867).

实验方案

Product Specific Protocols
WB protocol for Alpha-1-Antitrypsin antibody 16382-1-APDownload protocol
IHC protocol for Alpha-1-Antitrypsin antibody 16382-1-APDownload protocol
IF protocol for Alpha-1-Antitrypsin antibody 16382-1-APDownload protocol
IP protocol for Alpha-1-Antitrypsin antibody 16382-1-APDownload protocol
FC protocol for Alpha-1-Antitrypsin antibody 16382-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell

Neutrophil elastase selectively kills cancer cells and attenuates tumorigenesis.

Authors - Chang Cui
View Article
humanWB

Nat Commun

Alpha-1 antitrypsin inhibits TMPRSS2 protease activity and SARS-CoV-2 infection.

Authors - Lukas Wettstein
View Article
mouseWB

Nat Commun

The RNA-binding protein vigilin regulates VLDL secretion through modulation of Apob mRNA translation.

Authors - Mehrpouya B Mobin
View Article
humanIF

Matrix Biol

Proteome-wide and matrisome-specific atlas of the human ovary computes fertility biomarker candidates and open the way for precision oncofertility.

Authors - Emna Ouni
View Article
humanIF

Int J Mol Sci

Vesicular Integral-Membrane Protein 36 Is Involved in the Selective Secretion of Fucosylated Proteins into Bile Duct-like Structures in HepG2 Cells

Authors - Mizuki Muranaka
View Article
humanWB

J Nutr Biochem

Characterization of dietary and herbal sourced natural compounds that modulate SEL1L-HRD1 ERAD activity and alleviate protein misfolding in the ER

Authors - Jifeng Yang
View Article