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验证数据展示

  • WB analysis of HeLa using 20265-1-AP

    WB analysis of HeLa using 20265-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 20265-1-AP (SERINC1 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 20265-1-AP

    IHC staining of human liver cancer using 20265-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 20265-1-AP (SERINC1 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human liver cancer using 20265-1-AP

    IHC staining of human liver cancer using 20265-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 20265-1-AP (SERINC1 antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HepG2 using 20265-1-AP

    IF Staining of HepG2 using 20265-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 20265-1-AP (SERINC1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

SERINC1 Polyclonal antibody

SERINC1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 20265-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

KIAA1253, SERINC1, serine incorporator 1, TDE1L, TDE2, TMS 2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, L02 cells
Positive IHC detected inhuman liver cancer tissue, human cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20265-1-AP targets SERINC1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 serine incorporator 1
别名 KIAA1253, SERINC1, serine incorporator 1, TDE1L, TDE2, TMS 2
计算分子量 51 kDa
观测分子量 65 kDa
GenBank蛋白编号NM_020755
基因名称 SERINC1
Gene ID (NCBI) 57515
RRIDAB_10693613
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NRX5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SERINC1, also named as KIAA1253, TDE1L and TDE2, belongs to the TDE1 family. SERINC1 enhances the incorporation of serine into phosphatidylserine and sphingolipids. The antibody is specific to SERINC1.

实验方案

Product Specific Protocols
WB protocol for SERINC1 antibody 20265-1-APDownload protocol
IHC protocol for SERINC1 antibody 20265-1-APDownload protocol
IF protocol for SERINC1 antibody 20265-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols