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SERCA2,ATP2A2 Polyclonal antibody

SERCA2,ATP2A2 Polyclonal Antibody for IF/ICC,IF-P, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC,IF-P, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 27311-1-AP

Print datasheet

Synonyms

ATP2A2, ATP2B, Calcium pump 2, DAR, DD, SERCA2, SERCA2,ATP2A2, SR Ca(2+) ATPase 2

验证数据展示

  • WB analysis using 27311-1-AP

    WB analysis using 27311-1-AP

    Boiled and unboiled mouse heart lysates were subjected to SDS PAGE followed by western blot with 27311-1-AP (SERCA2,ATP2A2 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse skeletal muscle using 27311-1-AP

    IHC staining of mouse skeletal muscle using 27311-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 27311-1-AP (SERCA2,ATP2A2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of C2C12 using 27311-1-AP

    IF Staining of C2C12 using 27311-1-AP

    Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using SERCA2,ATP2A2 antibody (27311-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

经过测试的应用

Positive WB detected inmouse heart tissue, HEK-293 cells, mouse heart unboiled, 37°C incubated HEK-293 cells
Positive IHC detected inmouse skeletal muscle tissue, mouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inC2C12 cells, mouse heart tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

27311-1-AP targets SERCA2,ATP2A2 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IF/ICC,IF-P, IHC, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse
Cited Reactivitymouse
Immunogen SERCA2,ATP2A2 fusion protein Ag26177 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ATPase, Ca++ transporting, cardiac muscle, slow twitch 2
Synonyms ATP2A2, ATP2B, Calcium pump 2, DAR, DD, SERCA2, SERCA2,ATP2A2, SR Ca(2+) ATPase 2
Calculated Molecular Weight 1042 aa, 115 kDa
Observed Molecular Weight110-115 kDa
GenBank Accession NumberBC035588
Gene Symbol ATP2A2
Gene ID (NCBI) 488
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP16615
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ATP2A2, also known as SERCA2 (Sarco/endoplasmic reticulum-type calcium transport ATPase 2), is a 110 kDa magnesium-dependent enzyme catalyzing the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Human SERCA2 gene gives rise to several alternatively spliced isoforms: SERCA2a, SERCA2b and SERCA2c. ATP2A2 is mainly expressed in the sarcoplasmic reticulum of muscle cells and the endoplasmic reticulum of most cell types.

实验方案

Product Specific Protocols
WB protocol for SERCA2,ATP2A2 antibody 27311-1-APDownload protocol
IHC protocol for SERCA2,ATP2A2 antibody 27311-1-APDownload protocol
IF protocol for SERCA2,ATP2A2 antibody 27311-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

JCI Insight

Critical role of Znhit1 for postnatal heart function and vacuolar cardiomyopathy.

Authors - Yingchao Shi
View Article