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验证数据展示

  • WB analysis using 67792-1-Ig (same clone as 67792-1-PBS)

    WB analysis using 67792-1-Ig (same clone as 67792-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67792-1-Ig (SERBP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67792-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 67792-1-Ig (same clone as 67792-1-PBS)

    IHC staining of human liver cancer using 67792-1-Ig (same clone as 67792-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67792-1-Ig (SERBP1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67792-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 67792-1-Ig (same clone as 67792-1-PBS)

    IHC staining of human liver cancer using 67792-1-Ig (same clone as 67792-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67792-1-Ig (SERBP1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67792-1-PBS in a different storage buffer formulation.

  • IF Staining of human liver cancer using 67792-1-Ig (same clone as 67792-1-PBS)

    IF Staining of human liver cancer using 67792-1-Ig (same clone as 67792-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using SERBP1 antibody (67792-1-Ig, Clone: 1D4H1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). DAPI (blue). This data was developed using the same antibody clone with 67792-1-PBS in a different storage buffer formulation.

  • Cytometric bead array sample test of MP51733-1

    Cytometric bead array sample test of MP51733-1

    Cytometric bead array sample test of MP51733-1, SERBP1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67792-1-PBS. Detection antibody: 67792-2-PBS.

  • IP experiment of HeLa using 67792-1-Ig (same clone as 67792-1-PBS)

    IP experiment of HeLa using 67792-1-Ig (same clone as 67792-1-PBS)

    IP result of anti-SERBP1 (IP:67792-1-Ig, 5ug; Detection:67792-1-Ig 1:2000) with HeLa cells lysate 1880 ug. This data was developed using the same antibody clone with 67792-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 67792-1-Ig (same clone as 67792-1-PBS)

    IP experiment of HeLa using 67792-1-Ig (same clone as 67792-1-PBS)

    IP result of anti-SERBP1 (IP:67792-1-Ig, 5ug; Detection:67792-1-Ig 1:2000) with HeLa cells lysate 1880 ug. This data was developed using the same antibody clone with 67792-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP51733-1

    Cytometric bead array standard curve of MP51733-1

    Cytometric bead array standard curve of MP51733-1, SERBP1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67792-1-PBS. Detection antibody: 67792-2-PBS. Standard:Ag30202. Range: 3.125-400 ng/mL.

SERBP1 Monoclonal antibody, PBS Only (Capture)

SERBP1 Monoclonal Antibody for WB, IHC, IF-P, IP, Cytometric bead array, Indirect ELISA
Cat No. 67792-1-PBS

产品说明书

CloneNo. 1D4H1

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig, canine

应用

WB, IHC, IF-P, IP, Cytometric bead array, Indirect ELISA

别名

1D4H1, CGI-55, CHD3IP, HABP4L, PAI RBP1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

67792-1-PBS targets SERBP1 as part of a matched antibody pair:

MP51733-1: 67792-1-PBS capture and 67792-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF-P, IP, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse, rat, pig, canine
免疫原

CatNo: Ag30202

Product name: Recombinant human SERBP1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 108-408 aa of BC008045

Sequence: RVGRRPDQQLQGEGKIIDRRPERRPPRERRFEKPLEEKGEGGEFSVDRPIIDRPIRGRGGLGRGRGGRGRGMGRGDGFDSRGKREFDRHSGSDRSSFSHYSGLKHEDKRGGSGSHNWGTVKDELTESPKYIQKQISYNYSDLDQSNVTEETPEGEEHHPVADTENKENEVEEVKEEGPKEMTLDEWKAIQNKDRAKVEFNIRKPNEGADGQWKKGFVLHKSKSEEAHAEDSVMDHHFRKPANDITSQLEINFGDLGRPGRGGRGGRGGRGRGGRPNRGSRTDKSSASAPDVDDPEAFPALA

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 SERPINE1 mRNA binding protein 1
别名 1D4H1, CGI-55, CHD3IP, HABP4L, PAI RBP1
计算分子量 45 kDa
观测分子量50 kDa
GenBank蛋白编号BC008045
基因名称 SERBP1
Gene ID (NCBI) 26135
RRIDAB_2918556
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ8NC51
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The plasminogen activator system (PA) plays an important role in invasion and metastasis of solid tumors. The PA Inhibitor type 1 (PAI-1) is the main physiologic regulator of plasminogen activation. PAI-RBP1 (PAI-1 mRNA Binding Protein 1, also named SERBP1), is an RNA-binding protein containing an RG-rich domain and an RGG box domain that might be methylated by protein arginine N-methyltransferases (PRMTs). SERBP1 was identified as an interacting partner of a Tudor domain containing protein TDRD3, and can localize to cytoplasmic stress granules (SGs).

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