验证数据展示
经过测试的应用
| Positive WB detected in | A375 cells, HepG2 cells, Jurkat cells, rat testis tissue, mouse testis tissue |
| Positive IHC detected in | human gliomas tissue, human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
13818-1-AP targets Septin 7 in WB, IHC, IF/ICC, CoIP, ELISA, PLA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IF, CoIP, PLA |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat, bovine |
| 免疫原 |
CatNo: Ag4907 Product name: Recombinant human SEPT7 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 110-417 aa of BC025987 Sequence: VIDYIDSKFEDYLNAESRVNRRQMPDNRVQCCLYFIAPSGHGLKPLDIEFMKRLHEKVNIIPLIAKADTLTPEECQQFKKQIMKEIQEHKIKIYEFPETDDEEENKLVKKIKDRLPLAVVGSNTIIEVNGKRVRGRQYPWGVAEVENGEHCDFTILRNMLIRTHMQDLKDVTNNVHYENYRSRKLAAVTYNGVDNNKNKGQLTKSPLAQMEEERREHVAKMKKMEMEMEQVFEMKVKEKVQKLKDSEAELQRRHEQMKKNLEAQHKELEEKRRQFEDEKANWEAQQRILEQQNSSRTLEKNKKKGKIF 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | septin 7 |
| 别名 | septin7, SEPT7A, SEPT7, SEPT 7, CDC3 |
| 计算分子量 | 51 kDa |
| 观测分子量 | 45-50 kDa |
| GenBank蛋白编号 | BC025987 |
| 基因名称 | Septin 7 |
| Gene ID (NCBI) | 989 |
| RRID | AB_2254298 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q16181 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Septins (SEPTs) are members of a conserved family of cytoskeletal GTPases involved in various cellular processes, including cytoskeleton organization, cytokinesis, and membrane dynamics. SEPT7 is a unique septin required for filament formation. It has also been reported to be involved in migration and invasion in various cancer cells, including breast cancer and glioma.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for Septin 7 antibody 13818-1-AP | Download protocol |
| IHC protocol for Septin 7 antibody 13818-1-AP | Download protocol |
| WB protocol for Septin 7 antibody 13818-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Cell Biol The septin cytoskeleton regulates natural killer cell lytic granule release.
| ||
Cell Rep Cdc42EP3/BORG2 and Septin Network Enables Mechano-transduction and the Emergence of Cancer-Associated Fibroblasts. | ||
MBio Role of Endothelial Cell Septin 7 in the Endocytosis of Candida albicans.
| ||
Development Sperm-borne miR-202 targets SEPT7 and regulates first cleavage of bovine embryos via cytoskeletal remodeling. |
