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Septin 11 Polyclonal antibody

Septin 11 Polyclonal Antibody for FC, IF/ICC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC, IF/ICC, IHC, IP, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 14672-1-AP

Print datasheet

Synonyms

SEPT11, septin 11, Septin11

验证数据展示

  • WB analysis of human brain using 14672-1-AP

    WB analysis of human brain using 14672-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 14672-1-AP (Septin 11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary using 14672-1-AP

    IHC staining of human ovary using 14672-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 14672-1-AP (Septin 11 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human ovary using 14672-1-AP

    IHC staining of human ovary using 14672-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 14672-1-AP (Septin 11 antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HeLa using 14672-1-AP

    IF Staining of HeLa using 14672-1-AP

    Immunofluorescent analysis of Hela cells, using Septin 11 antibody 14672-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

  • IP experiment of mouse brain using 14672-1-AP

    IP experiment of mouse brain using 14672-1-AP

    IP result of anti-Septin 11 (IP:14672-1-AP, 4ug; Detection:14672-1-AP 1:500) with mouse brain tissue lysate 3440ug.

  • FC experiment of HeLa using 14672-1-AP

    FC experiment of HeLa using 14672-1-AP

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human Septin 11 (14672-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inhuman brain tissue, HeLa cells, HT-1080 cells, mouse brain tissue, mouse colon tissue, mouse small intestine tissue, SH-SY5Y cells
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman ovary tissue, human kidney tissue, human lung tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHela cells, HepG2 cells
Positive FC detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14672-1-AP targets Septin 11 in WB, IP, IF, FC, RIP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC, IF/ICC, IHC, IP, WB, ELISA
Cited ApplicationsWB, IF, RIP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen Septin 11 fusion protein Ag6342 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name septin 11
Synonyms SEPT11, septin 11, Septin11
Calculated Molecular Weight 49 kDa
Observed Molecular Weight 48 kDa
GenBank Accession NumberBC063615
Gene Symbol SEPT11
Gene ID (NCBI) 55752
RRIDAB_2301682
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NVA2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for Septin 11 antibody 14672-1-APDownload protocol
IHC protocol for Septin 11 antibody 14672-1-APDownload protocol
IF protocol for Septin 11 antibody 14672-1-APDownload protocol
IP protocol for Septin 11 antibody 14672-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseRIP

Cancer Res

CircFOXK2 Promotes Growth and Metastasis of Pancreatic Ductal Adenocarcinoma by Complexing with RNA Binding Proteins and Sponging MiR-942.

Authors - Chi Hin Wong
View Article
mouseIF

Cytoskeleton (Hoboken)

Characterization of septin expression in normal and fibrotic kidneys.

Authors - Katharina Neubauer
View Article
humanIF,WB

Cell Rep

An anillin-CIN85-SEPT9 complex promotes intercellular bridge maturation required for successful cytokinesis

Authors - Thomas C Panagiotou
View Article
humanWB

Cancers (Basel)

Forchlorfenuron-Induced Mitochondrial Respiration Inhibition and Metabolic Shifts in Endometrial Cancer

Authors - Kyukwang Kim
View Article