验证数据展示
经过测试的应用
| Positive WB detected in | human brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human ovary tissue, human lung tissue, rat spleen tissue, mouse liver tissue, mouse stomach tissue, human spleen tissue, human kidney tissue, rat liver tissue, human stomach cancer tissue, rat stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | Hela cells, HepG2 cells |
| Positive FC (Intra) detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy SEPT11 Ready-To-Use IHC Kit. SEPT11 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 647 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
14672-1-AP targets Septin 11 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description |
| 文献引用应用 | WB, IF, IP, CoIP, RIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag6342 Product name: Recombinant human SEPT11 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-150 aa of BC063615 Sequence: MAVAVGRPSNEELRNLSLSGHVGFDSLPDQLVNKSTSQGFCFNILCVGETGIGKSTLMDTLFNTKFESDPATHNEPGVRLKARSYELQESNVRLKLTIVDTVGFGDQINKDDSYKPIVEYIDAQFEAYLQEELKIKRSLFNYHDTRIHAC 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | septin 11 |
| 别名 | SEPT11, SEPTIN11, Septin-11 |
| 计算分子量 | 49 kDa |
| 观测分子量 | 48 kDa |
| GenBank蛋白编号 | BC063615 |
| 基因名称 | Septin 11 |
| Gene ID (NCBI) | 55752 |
| RRID | AB_2301682 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9NVA2 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Septin 11 antibody 14672-1-AP | Download protocol |
| IP protocol for Septin 11 antibody 14672-1-AP | Download protocol |
| IHC protocol for Septin 11 antibody 14672-1-AP | Download protocol |
| IF protocol for Septin 11 antibody 14672-1-AP | Download protocol |
| FC protocol for Septin 11 antibody 14672-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cancer Res CircFOXK2 Promotes Growth and Metastasis of Pancreatic Ductal Adenocarcinoma by Complexing with RNA Binding Proteins and Sponging MiR-942. | ||
Nat Commun Quantitative determination of the spatial distribution of components in single cells with CellDetail | ||
Brain Septin multimer autoantibodies in severe motor neuropathy mimicking lower motor neuron disease. | ||
Cell Death Dis Septin11 promotes hepatocellular carcinoma cell motility by activating RhoA to regulate cytoskeleton and cell adhesion | ||
Cell Rep An anillin-CIN85-SEPT9 complex promotes intercellular bridge maturation required for successful cytokinesis | ||
Cancers (Basel) Forchlorfenuron-Induced Mitochondrial Respiration Inhibition and Metabolic Shifts in Endometrial Cancer |
