验证数据展示
经过测试的应用
| Positive WB detected in | human brain tissue, HeLa cells, HT-1080 cells, mouse brain tissue, mouse colon tissue, mouse small intestine tissue, SH-SY5Y cells |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human ovary tissue, human kidney tissue, human lung tissue, human spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | Hela cells, HepG2 cells |
| Positive FC (Intra) detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy SEPT11 Ready-To-Use IHC Kit. SEPT11 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 647 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
14672-1-AP targets Septin 11 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description |
| 文献引用应用 | WB, IF, IP, CoIP, RIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag6342 Product name: Recombinant human SEPT11 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-150 aa of BC063615 Sequence: MAVAVGRPSNEELRNLSLSGHVGFDSLPDQLVNKSTSQGFCFNILCVGETGIGKSTLMDTLFNTKFESDPATHNEPGVRLKARSYELQESNVRLKLTIVDTVGFGDQINKDDSYKPIVEYIDAQFEAYLQEELKIKRSLFNYHDTRIHAC 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | septin 11 |
| 别名 | SEPT11, SEPTIN11, Septin-11 |
| 计算分子量 | 49 kDa |
| 观测分子量 | 48 kDa |
| GenBank蛋白编号 | BC063615 |
| 基因名称 | Septin 11 |
| Gene ID (NCBI) | 55752 |
| RRID | AB_2301682 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9NVA2 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for Septin 11 antibody 14672-1-AP | Download protocol |
| IHC protocol for Septin 11 antibody 14672-1-AP | Download protocol |
| IP protocol for Septin 11 antibody 14672-1-AP | Download protocol |
| WB protocol for Septin 11 antibody 14672-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cancer Res CircFOXK2 Promotes Growth and Metastasis of Pancreatic Ductal Adenocarcinoma by Complexing with RNA Binding Proteins and Sponging MiR-942. | ||
Cytoskeleton (Hoboken) Characterization of septin expression in normal and fibrotic kidneys. | ||
Cell Rep An anillin-CIN85-SEPT9 complex promotes intercellular bridge maturation required for successful cytokinesis | ||
Cancers (Basel) Forchlorfenuron-Induced Mitochondrial Respiration Inhibition and Metabolic Shifts in Endometrial Cancer | ||
Cell Death Dis Septin11 promotes hepatocellular carcinoma cell motility by activating RhoA to regulate cytoskeleton and cell adhesion | ||
Nat Commun Quantitative determination of the spatial distribution of components in single cells with CellDetail |
