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验证数据展示

  • WB analysis of mouse liver using 11551-1-AP

    WB analysis of mouse liver using 11551-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 11551-1-AP (SLA/LP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse liver using 11551-1-AP

    IP experiment of mouse liver using 11551-1-AP

    IP result of anti-SLA/LP (IP:11551-1-AP, 3ug; Detection:11551-1-AP 1:800) with mouse liver tissue lysate 6000ug.

  • IHC staining of human stomach using 11551-1-AP

    IHC staining of human stomach using 11551-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 11551-1-AP (SLA/LP antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

SLA/LP Polyclonal antibody

SLA/LP Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 11551-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IP, ELISA

别名

Selenocysteine synthase, Sec synthase, O-phosphoseryl-tRNA(Sec) selenium transferase, LP, Liver-pancreas antigen

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse liver tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

11551-1-AP targets SLA/LP in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IP, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原

CatNo: Ag2097

Product name: Recombinant human SEPSECS protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 117-422 aa of BC023539

Sequence: DELRTDLKAVEAKVQELGPDCILCIHSTTSCFAPRVPDRLEELAVICANYDIPHIVNNAYGVQSSKCMHLIQQGARVGRIDAFVQSLDKNFMVPVGGAIIAGFNDSFIQEISKMYPGRASASPSLDVLITLLSLGSNGYKKLLKERKEMFSYLSNQIKKLSEAYNERLLHTPHNPISLAMTLKTLDEHRDKAVTQLGSMLFTRQVSGARVVPLGSMQTVSGYTFRGFMSHTNNYPCAYLNAASAIGMKMQDVDLFIKRLDRCLKAVRKERSKESDDNYDKTEDVDIEEMALKLDNVLLDTYQDASS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 Sep (O-phosphoserine) tRNA:Sec (selenocysteine) tRNA synthase
别名 Selenocysteine synthase, Sec synthase, O-phosphoseryl-tRNA(Sec) selenium transferase, LP, Liver-pancreas antigen
计算分子量 501 aa, 56 kDa
观测分子量 56 kDa
GenBank蛋白编号BC023539
基因名称 SEPSECS
Gene ID (NCBI) 51091
RRIDAB_2186853
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9HD40
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SLA/LP antibody 11551-1-APDownload protocol
IHC protocol for SLA/LP antibody 11551-1-APDownload protocol
IP protocol for SLA/LP antibody 11551-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nature

Global landscape of HIV-human protein complexes.

Authors - Stefanie Jäger
View Article