验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, NIH/3T3 cells |
| Positive IHC detected in | mouse skeletal muscle tissue, rat skeletal muscle tissue, mouse uterus tissue, mouse lung tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy SEPN1 Ready-To-Use IHC Kit. SEPN1 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 10 publications below |
| IHC | See 1 publications below |
产品信息
55333-1-AP targets SEPN1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
fusion protein 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | selenoprotein N, 1 |
| 别名 | MDRS1, RSMD1, RSS, SELENON, Selenoprotein N |
| 计算分子量 | 66 kDa |
| 观测分子量 | 70 kDa |
| GenBank蛋白编号 | NM_020451 |
| 基因名称 | SEPN1 |
| Gene ID (NCBI) | 57190 |
| RRID | AB_2881308 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9NZV5 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SEPN1 (Selenoprotein N, 1) is a selenoprotein that plays a crucial role in maintaining cellular redox homeostasis and calcium homeostasis. It is encoded by the SEPN1 gene and is widely expressed in various tissues, particularly in skeletal muscle. (PMID: 32661288)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SEPN1 antibody 55333-1-AP | Download protocol |
| IHC protocol for SEPN1 antibody 55333-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Front Endocrinol (Lausanne) Changes and related factors of blood CCN1 levels in diabetic patients | ||
Antioxidants (Basel) Dietary Marginal and Excess Selenium Increased Triglycerides Deposition, Induced Endoplasmic Reticulum Stress and Differentially Influenced Selenoproteins Expression in the Anterior and Middle Intestines of Yellow Catfish Pelteobagrus fulvidraco. | ||
Sci Rep Damage to the myogenic differentiation of C2C12 cells by heat stress is associated with up-regulation of several selenoproteins. | ||
Mar Drugs Combination of Fish Oil and Selenium Enhances Anticancer Efficacy and Targets Multiple Signaling Pathways in Anti-VEGF Agent Treated-TNBC Tumor-Bearing Mice. | ||
Biol Trace Elem Res Activation of the De Novo Serine Synthesis Pathway and Disruption of Insulin Signaling Induced by Supplemental SeMet in Vitro | ||
