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验证数据展示

  • WB analysis of HepG2 using 16635-1-AP

    WB analysis of HepG2 using 16635-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 16635-1-AP (SEPHS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis using 16635-1-AP

    WB analysis using 16635-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16635-1-AP (SEPHS1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 16635-1-AP

    IHC staining of human lung cancer using 16635-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 16635-1-AP (SEPHS1 antibody) at dilution of 1:100 (under 10x lens).

    Ready-to-use IHC kits available with SEPHS1 antibody included
  • IHC staining of human lung cancer using 16635-1-AP

    IHC staining of human lung cancer using 16635-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 16635-1-AP (SEPHS1 antibody) at dilution of 1:100 (under 40x lens).

    Ready-to-use IHC kits available with SEPHS1 antibody included
  • IF Staining of human placenta using 16635-1-AP

    IF Staining of human placenta using 16635-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using SEPHS1 antibody (16635-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of human placenta using 16635-1-AP

    IF Staining of human placenta using 16635-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using SEPHS1 antibody (16635-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Featured Product
  • KD/KO验证

SEPHS1 Polyclonal antibody

SEPHS1 Polyclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 16635-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, ELISA

别名

selenophosphate synthetase 1, Selenophosphate synthase 1, Selenium donor protein 1, Selenide, water dikinase 1, SELD

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inJurkat cells, HepG2 cells, mouse liver tissue, T-47D cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman placenta tissue
Planning an IHC experiment? We recommend our IHCeasy SEPHS1 Ready-To-Use IHC Kit. SEPHS1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16635-1-AP targets SEPHS1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原

CatNo: Ag9978

Product name: Recombinant human SEPHS1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-392 aa of BC000941

Sequence: MSTRESFNPESYELDKSFRLTRFTELKGTGCKVPQDVLQKLLESLQENHFQEDEQFLGAVMPRLGIGMDTCVIPLRHGGLSLVQTTDYIYPIVDDPYMMGRIACANVLSDLYAMGVTECDNMLMLLGVSNKMTDRERDKVMPLIIQGFKDAAEEAGTSVTGGQTVLNPWIVLGGVATTVCQPNEFIMPDNAVPGDVLVLTKPLGTQVAVAVHQWLDIPEKWNKIKLVVTQEDVELAYQEAMMNMARLNRTAAGLMHTFNAHAATDITGFGILGHAQNLAKQQRNEVSFVIHNLPVLAKMAAVSKACGNMFGLMHGTCPETSGGLLICLPREQAARFCAEIKSPKYGEGHQAWIIGIVEKGNRTARIIDKPRIIEVAPQVATQNVNPTPGATS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 selenophosphate synthetase 1
别名 selenophosphate synthetase 1, Selenophosphate synthase 1, Selenium donor protein 1, Selenide, water dikinase 1, SELD
计算分子量 43 kDa
观测分子量 43 kDa
GenBank蛋白编号BC000941
基因名称 SEPHS1
Gene ID (NCBI) 22929
RRIDAB_10603472
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP49903
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Selenophosphate synthetase 1 (SEPHS1) has been implicated in the maintenance of redox homeostasis in chondrocytes and its deficiency plays a causal role in the progression of osteoarthritis (PMID: 36202216). SEPHS1 downregulation in human umbilical vein cells (HUVECs) led to cell death rather than the inhibition of cell proliferation (PMID: 33622411).

实验方案

Product Specific Protocols
IF protocol for SEPHS1 antibody 16635-1-APDownload protocol
IHC protocol for SEPHS1 antibody 16635-1-APDownload protocol
WB protocol for SEPHS1 antibody 16635-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
WB

Biomaterials

H2Se-evolving bio-heterojunctions promote cutaneous regeneration in infected wounds by inhibiting excessive cellular senescence

Authors - Fan Yang
View Article
humanWB,IHC

Exp Hematol Oncol

SEPHS1 promotes SMAD2/3/4 expression and hepatocellular carcinoma cells invasion.

Authors - Shu Yang
  • KD Validated
View Article
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