验证数据展示
经过测试的应用
| Positive WB detected in | MCF-7 cells, MDA-MB-231 cells, mouse brain tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
22571-1-AP targets SEMA6B in WB, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse |
| 免疫原 | SEMA6B fusion protein Ag16707 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6B |
| 别名 | SEM SEMA Y, SEMA VIB, Sema Z, SEMAN, Semaphorin 6B |
| 计算分子量 | 95, 74 kDa |
| 观测分子量 | 70 kDa |
| GenBank蛋白编号 | BC142617 |
| 基因名称 | SEMA6B |
| Gene ID (NCBI) | 10501 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen Affinity purified |
| UNIPROT ID | Q9H3T3 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Semaphorins (SEMAs) play critical roles in inflammation, angiogenesis, apoptosis, fibrosis, bone remodeling, and cell invasion. SEMA6B, the sixth member of the SEMA family, has shown significant correlations with the stage of liver fibrosis in hepatitis C virus-infected patients. SEMA6B is also involved in inflammatory responses and serves as a biomarker for macrophage-specific genes. Additionally, the level of SEMA6B in circulating monocytes has predictive value for cardiovascular disease. Also, SEMA6B can be a valuable biomarker for diagnosing HBV-ACLF12 (PMID: 39267780).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SEMA6B antibody 22571-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |