验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
33577-1-AP targets SEMA6A in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag40550 Product name: Recombinant human SEMA6A protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 400-649 aa of BC032619 Sequence: EAVPSIFNRPWFLRTMVRYRLTKIAVDTAAGPYQNHTVVFLGSEKGIILKFLARIGNSGFLNDSLFLEEMSVYNSEKCSYDGVEDKRIMGMQLDRASSSLYVAFSTCVIKVPLGRCERHGKCKKTCIASRDPYCGWIKEGGACSHLSPNSRLTFEQDIERGNTDGLGDCHNSFVALNGHSSSLLPSTTTSDSTAQEGYESRGGMLDWKHLLDSPDSTDPLGAVSSHNHQDKKGVIRESYLKGHDQLVPVT 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6A |
| 别名 | KIAA1368, Sema VIA, SEMA6A1, SEMAQ |
| 计算分子量 | 1030 aa, 114 kDa |
| 观测分子量 | 114 kDa |
| GenBank蛋白编号 | BC032619 |
| 基因名称 | SEMA6A |
| Gene ID (NCBI) | 57556 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | Q9H2E6 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Semaphorin 6A (SEMA6A) is a member of the semaphorin family. In humans it is encoded on chromosome 5 and is expressed most strongly in the developing and adult nervous system, but it is also present in heart, kidney, adrenal gland and placenta. SEMA6A overexpression alleviated CRC progression by inhibiting tumor growth and metastasis both in vivo and in vitro (PMID: 41259456).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SEMA6A antibody 33577-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
