验证数据展示
经过测试的应用
| Positive WB detected in | COLO 320 cells, HeLa cells, SH-SY5Y cells, SW480 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12279-1-AP targets SEMA4G in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 | SEMA4G fusion protein Ag2928 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4G |
| 别名 | KIAA1619, Semaphorin-4G |
| 计算分子量 | 91 kDa |
| 观测分子量 | 105 kDa |
| GenBank蛋白编号 | BC020960 |
| 基因名称 | SEMA4G |
| Gene ID (NCBI) | 57715 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9NTN9 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Semaphorins constitute a large and growing gene family, several members of which are axon guidance molecules. SEMA4G, a novel class IV member of the semaphorin gene family, located on mouse chromosome 19. SEMA4G is expressed early in development in the brain, spinal cord, and several sensory organs as well as specific populations of projection neurons, compatible with the well-established function of semaphorins as axon guidance molecules.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SEMA4G antibody 12279-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |