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SEMA4F Polyclonal antibody

SEMA4F Polyclonal Antibody for IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IP, WB,ELISA

Conjugate

Unconjugated

Cat No : 12474-1-AP

Print datasheet

Synonyms

M SEMA, m Sema M, PRO2353, Sema M, Sema W, SEMA4F, SEMAM, Semaphorin 4F, Semaphorin M, Semaphorin W, SEMAW

验证数据展示

  • WB analysis of mouse lung using 12474-1-AP

    WB analysis of mouse lung using 12474-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 12474-1-AP (SEMA4F antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • IP experiment of HepG2 using 12474-1-AP

    IP experiment of HepG2 using 12474-1-AP

    IP result of anti-SEMA4F (IP:12474-1-AP, 4ug; Detection:12474-1-AP 1:500) with HepG2 cells lysate 3600ug.

经过测试的应用

Positive WB detected inmouse lung tissue, HepG2 cells, human liver tissue, mouse brain tissue, Y79 cells
Positive IP detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12474-1-AP targets SEMA4F in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IP, WB,ELISA
Tested Reactivity human, mouse, rat
Immunogen SEMA4F fusion protein Ag3082 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4F
Synonyms M SEMA, m Sema M, PRO2353, Sema M, Sema W, SEMA4F, SEMAM, Semaphorin 4F, Semaphorin M, Semaphorin W, SEMAW
Calculated Molecular Weight 615 aa, 66 kDa
Observed Molecular Weight 66 kDa, 84 kDa
GenBank Accession NumberBC018361
Gene Symbol SEMA4F
Gene ID (NCBI) 10505
RRIDAB_2185847
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO95754
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SEMA4F antibody 12474-1-APDownload protocol
IP protocol for SEMA4F antibody 12474-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols