验证数据展示
经过测试的应用
Positive WB detected in | mouse lung tissue, HepG2 cells, human liver tissue, mouse brain tissue, Y79 cells |
Positive IP detected in | HepG2 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:500-1:2000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
12474-1-AP targets SEMA4F in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, IP, ELISA Application Description |
经测试反应性 | human, mouse, rat |
免疫原 | SEMA4F fusion protein Ag3082 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4F |
别名 | M SEMA, m Sema M, PRO2353, Sema M, Sema W, SEMA4F, SEMAM, Semaphorin 4F, Semaphorin M, Semaphorin W, SEMAW |
计算分子量 | 615 aa, 66 kDa |
观测分子量 | 66 kDa, 84 kDa |
GenBank蛋白编号 | BC018361 |
基因名称 | SEMA4F |
Gene ID (NCBI) | 10505 |
RRID | AB_2185847 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | O95754 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
Product Specific Protocols | |
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WB protocol for SEMA4F antibody 12474-1-AP | Download protocol |
IP protocol for SEMA4F antibody 12474-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |