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验证数据展示

  • IHC staining of human colon cancer using 29191-1-AP

    IHC staining of human colon cancer using 29191-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29191-1-AP (SEMA4B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon cancer using 29191-1-AP

    IHC staining of human colon cancer using 29191-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29191-1-AP (SEMA4B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

SEMA4B Polyclonal antibody

SEMA4B Polyclonal Antibody for IHC, ELISA
Cat No. 29191-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human

应用

IHC, ELISA

别名

KIAA1745, SEMA4B, SEMAC, SemC

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29191-1-AP targets SEMA4B in IHC, ELISA applications and shows reactivity with Human samples.

经测试应用 IHC, ELISA Application Description
经测试反应性 Human
免疫原 SEMA4B fusion protein Ag30422 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4B
别名 KIAA1745, SEMA4B, SEMAC, SemC
GenBank蛋白编号BC110853
基因名称 SEMA4B
Gene ID (NCBI) 10509
RRIDAB_3086102
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SEMA4B (Semaphorin 4B), belonging to the Class SEMA4, is a transmembrane homodimer glycoprotein. Lots of SEMA4 family members have been implicated in the formation and progression of tumors due to (PMID: 35676688). SEMA4B inhibits the invasion of non-small cell lung cancer (NSCLC) through PI3K-dependent suppression of MMP9 activation (PMID: 25746385).

实验方案

Product Specific Protocols
IHC protocol for SEMA4B antibody 29191-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols