验证数据展示
经过测试的应用
| Positive IHC detected in | mouse pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
30638-1-AP targets SEMA3C in IHC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | IHC, ELISA Application Description |
| 经测试反应性 | human, mouse |
| 免疫原 |
CatNo: Ag32426 Product name: Recombinant human SEMA3C protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 560-751 aa of BC030690 Sequence: PLTQCRGFNLKAYRNAAEIVQYGVKNNTTFLECAPKSPQASIKWLLQKDKDRRKEVKLNERIIATSQGLLIRSVQGSDQGLYHCIATENSFKQTIAKINFKVLDSEMVAVVTDKWSPWTWASSVRALPFHPKDIMGAFSHSEMQMINQYCKDTRQQHQQGDESQKMRGDYGKLKALINSRKSRNRRNQLPES 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3C |
| 别名 | Sema E, SEMAE, Semaphorin 3C, Semaphorin E, Semaphorin-3C |
| 计算分子量 | 85 kDa |
| GenBank蛋白编号 | BC030690 |
| 基因名称 | SEMA3C |
| Gene ID (NCBI) | 10512 |
| RRID | AB_3742361 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q99985 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SEMA3C (Semaphorin 3C) is associated with tumor progression and poor prognosis across multiple tumor types, including pancreatic, gastric, prostate, and breast cancer, as well as glioma. It is worth noting that full-length SEMA3C has also been reported as a tumor suppressor factor by suppressing tumor lymphangiogenesis and metastasis (PMID: 31649890).
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for SEMA3C antibody 30638-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
