验证数据展示
经过测试的应用
| Positive WB detected in | Human peripheral blood platelets |
| Positive IHC detected in | human spleen tissue, human tonsillitis tissue, human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human tonsillitis tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 7 publications below |
| IF | See 4 publications below |
产品信息
13304-1-AP targets P-selectin/CD62P in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF-P, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag4124 Product name: Recombinant human SELP protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 441-789 aa of BC028067 Sequence: VCQALQCQDLPVPNEARVNCSHPFGAFRYQSVCSFTCNEGLLLVGASVLQCLATGNWNSVPPECQAIPCTPLLSPQNGTMTCVQPLGSSSYKSTCQFICDEGYSLSGPERLDCTRSGRWTDSPPMCEAIKCPELFAPEQGSLDCSDTRGEFNVGSTCHFSCNNGFKLEGPNNVECTTSGRWSATPPTCKGIASLPTPGVQCPALTTPGQGTMYCRHHPGTFGFNTTCYFGCNAGFTLIGDSTLSCRPSGQWTAVTPACRAVKCSELHVNKPIAMNCSNLWGNFSYGSICSFHCLEGQLLNGSAQTACQENGHWSTTVPTCQDDGKCPLNPHSHLGTYGVFTNAAFDPSP 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | selectin P (granule membrane protein 140kDa, antigen CD62) |
| 别名 | P-selectin / CD62P, CD62P, P-selectin, AK4, CD62 antigen-like family member P |
| 计算分子量 | 830 aa, 91 kDa |
| 观测分子量 | 140 kDa |
| GenBank蛋白编号 | BC028067 |
| 基因名称 | CD62P |
| Gene ID (NCBI) | 6403 |
| RRID | AB_2877937 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P16109 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
P-selectin, also known as GMP140 or CD62P, is a transmembrane glycoprotein that mediates the interaction of activated endothelial cells or platelets with leukocytes. It is an adhesion molecule involved in the pathogenesis of inflammation, thrombosis, and oncogenesis. P-selectin is stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. Upon cell activation by agonists, P-selectin is transported rapidly to the cell surface.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for P-selectin/CD62P antibody 13304-1-AP | Download protocol |
| IHC protocol for P-selectin/CD62P antibody 13304-1-AP | Download protocol |
| WB protocol for P-selectin/CD62P antibody 13304-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun Ubiquitin ligase CHFR mediated degradation of VE-cadherin through ubiquitylation disrupts endothelial adherens junctions | ||
Adv Sci (Weinh) Elevator-Like Hollow Channels in Porous Scaffolds Accelerate Vascularized Bone Regeneration via NETs-Fibrin-Mediated Macrophage Recruitment. | ||
Small Platelet Membrane Coated Cu9S8-SNAP for Targeting NIR-II Mild Photothermal Enhanced Chemodynamic/Gas Therapy of Triple-Negative Breast Cancer | ||
Int J Nanomedicine Bio-Inspired Nanodelivery Platform: Platelet Membrane-Cloaked Genistein Nanosystem for Targeted Lung Cancer Therapy | ||
Int J Biol Macromol Platelet-membrane camouflaged cerium nanoparticle-embedded gelatin methacryloyl hydrogel for accelerated diabetic wound healing | ||
Int J Biol Macromol Fucoidan-based smart micelles enable P-selectin-targeted and ROS-responsive delivery of mitochonic acid 5 for mitochondrial functional improvement in acute lung injury. |
