验证数据展示
经过测试的应用
| Positive WB detected in | K-562 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 4 publications below |
产品信息
12561-1-AP targets SELK in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | SELK fusion protein Ag3143 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | selenoprotein K |
| 别名 | Selenoprotein K, HSPC297, HSPC030 |
| 计算分子量 | 94 aa, 10 kDa |
| 观测分子量 | 10 kDa |
| GenBank蛋白编号 | BC013162 |
| 基因名称 | SELK |
| Gene ID (NCBI) | 58515 |
| RRID | AB_2186964 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9Y6D0 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Selenoprotein K (SelK), a single-pass transmembrane protein that resides in the endoplasmic reticulum (ER) membrane, which has been reported to an emerging role of playing a part in protein palmitoylation. Human SelK is a 94-amino-acid protein with Sec residing at the penultimate position. It has been shown that SelK is expressed predominantly in the heart and skeletal muscle, but other tissues, such as the liver, pancreas, and placenta, also have detectable levels of SelK.(PMID: 36252341)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SELK antibody 12561-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Biochem Biophys Res Commun Knockdown of Trnau1ap inhibits the proliferation and migration of NIH3T3, JEG-3 and Bewo cells via the PI3K/Akt signaling pathway. | ||
Front Pharmacol Prominent Effects of Berbamine Hydrochloride on Alzheimer's Disease Model Mice. | ||
Cell Mol Life Sci Selenium-SelK-GPX4 axis protects nucleus pulposus cells against mechanical overloading-induced ferroptosis and attenuates senescence of intervertebral disc | ||
Redox Rep Selenium participates in the formation of kidney stones by alleviating endoplasmic reticulum stress and apoptosis of renal tubular epithelial cells
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