E-selectin / CD62E Polyclonal antibody

E-selectin / CD62E Polyclonal Antibody for FC, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC, IHC, ELISA and More (2)

Conjugate

Unconjugated

Cat No : 20894-1-AP

Print datasheet

Synonyms

CD62E, ELAM, ELAM 1, ELAM1, ESEL, E-selectin, E-selectin /CD62E, LECAM2, SELE, selectin E



经过测试的应用

Positive IHC detected inhuman tonsillitis tissue, human appendicitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inHUVEC cells

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20894-1-AP targets E-selectin / CD62E in WB, IHC, IF, FC, ELISA applications and shows reactivity with human samples.

Tested Applications FC, IHC, ELISA
Cited Applications IF, IHC, WB
Tested Reactivity human
Cited Reactivityhuman
Immunogen E-selectin / CD62E fusion protein Ag14944 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name selectin E
Synonyms CD62E, ELAM, ELAM 1, ELAM1, ESEL, E-selectin, E-selectin /CD62E, LECAM2, SELE, selectin E
Calculated Molecular Weight 610 aa, 67 kDa
GenBank Accession NumberBC142711
Gene Symbol SELE
Gene ID (NCBI) 6401
RRIDAB_10755287
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP16581
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

E-selectin, also known as ELAM-1 or CD62E, is a member of the selectin family of adhesion molecules that also includes L-selectin and P-selectin. E-selectin is an inducible endothelial cell surface molecule. Its expression on endothelial cells is transcriptionally upregulated by various proinflammatory substances such as IL-1, TNFα and lipopolysaccharide (LPS). Besides, it can be secreted by endothelial cells, and can be detected in a soluble form (sE-selectin) in serum. During inflammation, E-selectin plays an important part in recruiting leukocytes to the site of injury. (PMID: 2827173; 1382417; 8943958)

实验方案

Product Specific Protocols
IHC protocol for E-selectin / CD62E antibody 20894-1-APDownload protocol
FC protocol for E-selectin / CD62E antibody 20894-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Adv Sci (Weinh)

Astrocyte-Derived Extracellular Vesicular miR-143-3p Dampens Autophagic Degradation of Endothelial Adhesion Molecules and Promotes Neutrophil Transendothelial Migration after Acute Brain Injury

Authors - Xun Wu
humanWB

Sci Adv

The support of genetic evidence for cardiovascular risk induced by antineoplastic drugs.

Authors - Hui Cui
humanIF

Biomater Sci

An E-selectin targeting and MMP-2-responsive dextran-curcumin polymeric prodrug for targeted therapy of acute kidney injury

Authors - Jing-Bo Hu
humanWB

Genomics

MIN score predicts primary response to infliximab/adalimumab and vedolizumab therapy in patients with inflammatory bowel diseases.

Authors - Yuan Shi
humanWB

Int J Mol Sci

Identification of Overexpressed Genes in Malignant Pleural Mesothelioma.

Authors - Federica Morani
humanWB

J Neuroinflammation

Downregulation of CD151 restricts VCAM-1 mediated leukocyte infiltration to reduce neurobiological injuries after experimental stroke.

Authors - Ceshu Gao