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验证数据展示

  • WB analysis using 28665-1-AP (same clone as 28665-1-PBS)

    WB analysis using 28665-1-AP (same clone as 28665-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 28665-1-AP (SEC63 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28665-1-PBS in a different storage buffer formulation.

  • WB analysis of HepG2 using 28665-1-AP (same clone as 28665-1-PBS)

    WB analysis of HepG2 using 28665-1-AP (same clone as 28665-1-PBS)

    WB result of SEC63 antibody (28665-1-AP; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SEC63 transfected HepG2 cells. This data was developed using the same antibody clone with 28665-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse pancreas using 28665-1-AP (same clone as 28665-1-PBS)

    IHC staining of mouse pancreas using 28665-1-AP (same clone as 28665-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 28665-1-AP (SEC63 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28665-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse pancreas using 28665-1-AP (same clone as 28665-1-PBS)

    IHC staining of mouse pancreas using 28665-1-AP (same clone as 28665-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 28665-1-AP (SEC63 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28665-1-PBS in a different storage buffer formulation.

  • IF Staining of HEK-293 using 28665-1-AP (same clone as 28665-1-PBS)

    IF Staining of HEK-293 using 28665-1-AP (same clone as 28665-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using SEC63 antibody (28665-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 28665-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

SEC63 Polyclonal antibody, PBS Only

SEC63 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 28665-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

DnaJ homolog subfamily C member 23, DNAJC23, SEC63L, Translocation protein SEC63 homolog

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

28665-1-PBS targets SEC63 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag30213

Product name: Recombinant human SEC63 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 559-728 aa of BC047221

Sequence: EAAVKEDEEEVSDKGSDSEEEETNRDSQSEKDDGSDRDSDREQDEKQNKDDEAEWQELQQSIQRKERALLETKSKITHPVYSLYFPEEKQEWWWLYIADRKEQTLISMPYHVCTLKDTEEVELKFPAPGKPGNYQYTVFLRSDSYMGLDQIKPLKLEVHEAKPVPENHPQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 SEC63 homolog (S. cerevisiae)
别名 DnaJ homolog subfamily C member 23, DNAJC23, SEC63L, Translocation protein SEC63 homolog
计算分子量 88 kDa
观测分子量 90 kDa
GenBank蛋白编号BC047221
基因名称 SEC63
Gene ID (NCBI) 11231
RRIDAB_2881189
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UGP8
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SEC63 is a integral ER membrane protein. SEC63 and SEC62 form a dimeric complex and play a central role in translocation of nascent and newly synthesized precursor polypeptides into the ER. Mutations in the gene encoding SEC63 cause polycystic liver disease in humans. The intact SEC63 protein is usually detected around 83-90 kDa, while smaller cleavage products can be observed by autophagy proteolysis (PMID: 25052096)

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