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验证数据展示

  • WB analysis using 85805-2-RR

    WB analysis using 85805-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85805-2-RR (SEC61B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis of HepG2 using 85805-2-RR

    WB analysis of HepG2 using 85805-2-RR

    WB result of SEC61B antibody (85805-2-RR; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SEC61B transfected HepG2 cells.

  • IF Staining of HeLa using 85805-2-RR

    IF Staining of HeLa using 85805-2-RR

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using SEC61B antibody (85805-2-RR, Clone: 250082D3 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity of 85805-2-RR

    Affinity of 85805-2-RR

    Biolayer interferometry (BLl) kinetic assays of 85805-2-RR against Human SEC61B were performed. The affinity constant is 28.9 pM.

  • KD/KO Validated

SEC61B Recombinant monoclonal antibody

SEC61B Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 85805-2-RR

产品说明书

CloneNo. 250082D3

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA

别名

Protein transport protein Sec61 subunit beta

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, HEK-293T cells, A431 cells, L02 cells, NIH/3T3 cells, C6 cells
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85805-2-RR targets SEC61B in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag7169

Product name: Recombinant human SEC61B protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-96 aa of BC001734

Sequence: MPGPTPSGTNVGSSGRSPSKAVAARAAGSTVRQRKNASCGTRSAGRTTSAGTGGMWRFYTEDSPGLKVGPVPVLVMSLLFIASVFMLHIWGKYTRS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 Sec61 beta subunit
别名 Protein transport protein Sec61 subunit beta
计算分子量 10 kDa
观测分子量 10-15 kDa
GenBank蛋白编号BC001734
基因名称 SEC61B
Gene ID (NCBI) 10952
RRIDAB_3744390
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP60468
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The heteromeric SEC61 complex is composed of alpha, beta and gamma subunits. Oligomers of the SEC61 complex form a transmembrane channel where proteins are translocated across and integrated into the ER membrane (PMID: 10212142). SEC61B is the beta subunit of the SEC61 complex. It has been reported that SEC61B facilitates cotranslational protein transport and interacts with the signal peptidase during translocation (PMID: 9585408).

实验方案

Product Specific Protocols
IF protocol for SEC61B antibody 85805-2-RRDownload protocol
WB protocol for SEC61B antibody 85805-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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