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验证数据展示

  • WB analysis of human brain using 14703-1-AP (same clone as 14703-1-PBS)

    WB analysis of human brain using 14703-1-AP (same clone as 14703-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 14703-1-AP (SEC6 antibody) at dilution of 1:86 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14703-1-PBS in a different storage buffer formulation.

  • WB analysis using 14703-1-AP (same clone as 14703-1-PBS)

    WB analysis using 14703-1-AP (same clone as 14703-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14703-1-AP (SEC6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14703-1-PBS in a different storage buffer formulation.

  • IHC staining of human gliomas using 14703-1-AP (same clone as 14703-1-PBS)

    IHC staining of human gliomas using 14703-1-AP (same clone as 14703-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 14703-1-AP (SEC6 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14703-1-PBS in a different storage buffer formulation.

  • IHC staining of human gliomas using 14703-1-AP (same clone as 14703-1-PBS)

    IHC staining of human gliomas using 14703-1-AP (same clone as 14703-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 14703-1-AP (SEC6 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14703-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 14703-1-AP (same clone as 14703-1-PBS)

    IF Staining of HepG2 using 14703-1-AP (same clone as 14703-1-PBS)

    Immunofluorescent analysis of HepG2 cells using 14703-1-AP (SEC6 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14703-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

SEC6 Polyclonal antibody, PBS Only

SEC6 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 14703-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

EXOC3, Exocyst complex component 3, Exocyst complex component Sec6, SEC6L1

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

14703-1-PBS targets SEC6 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag6383

Product name: Recombinant human SEC6 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 381-745 aa of BC064569

Sequence: DTYMSTLTSNIIAWLRKALETDKKDWVKETEPEADQDGYYQTTLPAIVFQMFEQNLQVAAQISEDLKTKVLVLCLQQMNSFLSRYKDEAQLYKEEHLRNRQHPHCYVQYMIAIINNCQTFKESIVSLKRKYLKNEVEEGVSPSQPSMDGILDAIAKEGCSGLLEEVFLDLEQHLNELMTKKWLLGSNAVDIICVTVEDYFNDFAKIKKPYKKRMTAEAHRRVVVEYLRAVMQKRISFRSPEERKEGAEKMVREAEQLRFLFRKLASGFGEDVDGYCDTIVAVAEVIKLTDPSLLYLEVSTLVSKYPDIRDDHIGALLAVRGDASRDMKQTIMETLEQGPAQASPSYVPLFKDIVVPSLNVAKLLK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 exocyst complex component 3
别名 EXOC3, Exocyst complex component 3, Exocyst complex component Sec6, SEC6L1
计算分子量 87 kDa
观测分子量 86 kDa
GenBank蛋白编号BC064569
基因名称 SEC6
Gene ID (NCBI) 11336
RRIDAB_2101542
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO60645
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Exoc3 (Sec6) is a component of the exocyst (Sec6/8) complex involved in vesicle trafficking at neural synapses and vesicle targeting in polarized epithelial cells. Though best characterized in yeast, the component proteins and functions of exocyst complex have been demonstrated to be highly conserved in higher eukaryotes. This complex is composed of eight proteins (Sec3, Sec5, Sec6, Sec8, Sec10, and Sec15, Exo70 and Exo84), with molecular weights ranging from 70 to 144 kDa.

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