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验证数据展示

  • WB analysis of HEK-293 using 66011-1-Ig (same clone as 66011-1-PBS)

    WB analysis of HEK-293 using 66011-1-Ig (same clone as 66011-1-PBS)

    WB result of SEC5/EXOC2 antibody (66011-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SEC5/EXOC2 transfected HEK-293 cells. This data was developed using the same antibody clone with 66011-1-PBS in a different storage buffer formulation.

  • WB analysis of mouse brain using 66011-1-Ig (same clone as 66011-1-PBS)

    WB analysis of mouse brain using 66011-1-Ig (same clone as 66011-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 66011-1-Ig (SEC5/EXOC2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66011-1-PBS in a different storage buffer formulation.

  • IF Staining of MDA-MB-231 using 66011-1-Ig (same clone as 66011-1-PBS)

    IF Staining of MDA-MB-231 using 66011-1-Ig (same clone as 66011-1-PBS)

    Immunostaining of exocyst protein Sec5 (66011-1-Ig, red) in MDA-MB-231 cells. The image was credited by Dr. Hae Lin Jang from Harvard Medical School. This data was developed using the same antibody clone with 66011-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 66011-1-Ig (same clone as 66011-1-PBS)

    IHC staining of human breast cancer using 66011-1-Ig (same clone as 66011-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66011-1-Ig (SEC5/EXOC2 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66011-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 66011-1-Ig (same clone as 66011-1-PBS)

    IHC staining of human breast cancer using 66011-1-Ig (same clone as 66011-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66011-1-Ig (SEC5/EXOC2 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66011-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse brain using 66011-1-Ig (same clone as 66011-1-PBS)

    IP experiment of mouse brain using 66011-1-Ig (same clone as 66011-1-PBS)

    IP result of anti-SEC5/EXOC2 (IP:66011-1-Ig, 5ug; Detection:66011-1-Ig 1:500) with mouse brain tissue lysate 3440ug. This data was developed using the same antibody clone with 66011-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 66011-1-Ig (same clone as 66011-1-PBS)

    IF Staining of HeLa using 66011-1-Ig (same clone as 66011-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SEC5/EXOC2 antibody (66011-1-Ig, Clone: 1C11G2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66011-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

SEC5/EXOC2 Monoclonal antibody, PBS Only

SEC5/EXOC2 Monoclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 66011-1-PBS

产品说明书

CloneNo. 1C11G2

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse, pig, rat

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

EXOC2, exocyst complex component 2, Exocyst complex component Sec5, SEC5, SEC5/EXOC2, SEC5L1, Sec5p

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

66011-1-PBS targets SEC5/EXOC2 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, pig, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, pig, rat
免疫原

CatNo: Ag17866

Product name: Recombinant human SEC5 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 456-806 aa of BC016918

Sequence: TIQDLILDLRVRCVMATLQHTAEEIKRLAEKEDWIVDNEGLTSLPCQFEQCIVCSLQSLKGVLECKPGEASVFQQPKTQEEVCQLSINIMQVFIYCLEQLSTKPDADIDTTHLSVDVSSPDLFGSIHEDFSLTSEQRLLIVLSNCCYLERHTFLNIAEHFEKHNFQGIEKITQVSMASLKELDQRLFENYIELKADPIVGSLEPGIYAGYFDWKDCLPPTGVRNYLKEALVNIIAVHAEVFTISKELVPRVLSKVIEAVSEELSRLMQCVSSFSKNGALQARLEICALRDTVAVYLTPESKSSFKQALEALPQLSSGADKKLLEELLNKFKSSMHLQLTCFQAASSTMMKT

种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 exocyst complex component 2
别名 EXOC2, exocyst complex component 2, Exocyst complex component Sec5, SEC5, SEC5/EXOC2, SEC5L1, Sec5p
计算分子量 924 aa, 104 kDa
观测分子量 100 kDa
GenBank蛋白编号BC016918
基因名称 SEC5
Gene ID (NCBI) 55770
RRIDAB_11042317
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ96KP1
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

EXOC2 (exocyst complex component 2), also known as SEC5 and SEC5L1, is a component of the exocyst complex, and is required to mediate RalB-dependent survival signals in transformed cells. The exocyst complex, composed of eight evolutionarily conserved subunits (SEC3, SEC5, SEC6, SEC8, SEC10, SEC15, EXO70, and EXO84), is involved in tethering post-Golgi secretory vesicles to specific plasma membrane domains. The gene of EXOC2 maps to chromosome 6p25.3, and encodes a 924-amino acid protein with an experimentally determined molecular mass of 95-100 kDa. EXOC2 mRNA is widely expressed with highest levels in brain and placenta.

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