验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, HepG2 cells, HeLa cells |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:8000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| CoIP | See 1 publications below |
产品信息
67409-1-Ig targets SEC24D in WB, IF/ICC, CoIP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 文献引用应用 | CoIP |
| 经测试反应性 | human |
| 文献引用反应性 | mouse |
| 免疫原 |
CatNo: Ag4657 Product name: Recombinant human SEC24D protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 783-1033 aa of BC035761 Sequence: TDALINFFAKSAFKAVLHQPLKVIREILVNQTAHMLACYRKNCASPSAASQLILPDSMKVLPVYMNCLLKNCVLLSRPEISTDERAYQRQLVMTMGVADSQLFFYPQLLPIHTLDVKSTMLPAAVRCSESRLSEEGIFLLANGLHMFLWLGVSSPPELIQGIFNVPSFAHINTDMTLLPEVGNPYSQQLRMIMGIIQQKRPYSMKLTIVKQREQPEMVFRQFLVEDKGLYGGSSYVDFLCCVHKEICQLLN 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2a |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | SEC24 family, member D (S. cerevisiae) |
| 别名 | SEC24-related protein D, SEC24 related protein D, KIAA0755, 3G5G9 |
| 计算分子量 | 1033 aa, 113 kDa |
| 观测分子量 | 113 kDa |
| GenBank蛋白编号 | BC035761 |
| 基因名称 | SEC24D |
| Gene ID (NCBI) | 9871 |
| RRID | AB_2882650 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | O94855 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
COPII-coated vesicles mediate anterograde transport from the ER to the Golgi apparatus. COPII is composed of three parts: two coat protein complexes (Sec23/24 complex and Sec13/31 complex) and one small GTP-binding protein, Sar1 (PMID: 9568718). Sec24 subunit of the coat complex has been implicated in cargo recognition (PMID: 17316621). In mammal cells there are four Sec24 isoforms, termed Sec24A, Sec24B, Sec24C, and Sec24D (PMID: 10329445).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for SEC24D antibody 67409-1-Ig | Download protocol |
| WB protocol for SEC24D antibody 67409-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
