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SEC24D Polyclonal antibody

SEC24D Polyclonal Antibody for IF/ICC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 13673-1-AP

Print datasheet

Synonyms

KIAA0755, SEC24 related protein D, SEC24D

验证数据展示

  • WB analysis using 13673-1-AP

    WB analysis using 13673-1-AP

    Mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 13673-1-AP (SEC24D antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas using 13673-1-AP

    IHC staining of human pancreas using 13673-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 13673-1-AP (SEC24D antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human pancreas using 13673-1-AP

    IHC staining of human pancreas using 13673-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 13673-1-AP (SEC24D antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 13673-1-AP

    IF Staining of HeLa using 13673-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 13673-1-AP (SEC24D antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inmouse pancreas tissue
Positive IHC detected inhuman pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

13673-1-AP targets SEC24D in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IF/ICC, IHC, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse
Cited Reactivitymouse
Immunogen SEC24D fusion protein Ag4657 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name SEC24 family, member D (S. cerevisiae)
Synonyms KIAA0755, SEC24 related protein D, SEC24D
Calculated Molecular Weight 1033 aa, 113 kDa
Observed Molecular Weight 113 kDa
GenBank Accession NumberBC035761
Gene Symbol SEC24D
Gene ID (NCBI) 9871
RRIDAB_2877968
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO94855
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

COPII-coated vesicles mediate anterograde transport from the ER to the Golgi apparatus. COPII is composed of three parts: two coat protein complexes (Sec23/24 complex and Sec13/31 complex) and one small GTP-binding protein, Sar1 (PMID: 9568718). Sec24 subunit of the coat complex has been implicated in cargo recognition (PMID: 17316621). In mammal cells there are four Sec24 isoforms, termed Sec24A, Sec24B, Sec24C, and Sec24D (PMID: 10329445).

实验方案

Product Specific Protocols
WB protocol for SEC24D antibody 13673-1-APDownload protocol
IHC protocol for SEC24D antibody 13673-1-APDownload protocol
IF protocol for SEC24D antibody 13673-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Nat Cell Biol

Manganese regulation of COPII condensation controls circulating lipid homeostasis

Authors - Xiao Wang
View Article