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验证数据展示

  • WB analysis of HeLa using 16073-1-AP

    WB analysis of HeLa using 16073-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 16073-1-AP (SEC24C antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 16073-1-AP

    IF Staining of HeLa using 16073-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 16073-1-AP (SEC24C antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

SEC24C Polyclonal antibody

SEC24C Polyclonal Antibody for WB, IF, ELISA
Cat No. 16073-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF, ELISA

别名

KIAA0079, SEC24 related protein C, SEC24C

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy SEC24C Ready-To-Use IHC Kit. SEC24C primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16073-1-AP targets SEC24C in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag9030

Product name: Recombinant human SEC24C protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 742-1094 aa of BC018928

Sequence: DVQKVVGFDAVMRVRTSTGIRAVDFFGAFYMSNTTDVELAGLDGDKTVTVEFKHDDRLNEESGALLQCALLYTSCAGQRRLRIHNLALNCCTQLADLYRNCETDTLINYMAKFAYRGVLNSPVKAVRDTLITQCAQILACYRKNCASPSSAGQLILPECMKLLPVYLNCVLKSDVLQPGAEVTTDDRAYVRQLVTSMDVTETNVFFYPRLLPLTKSPVESTTEPPAVRASEERLSNGDIYLLENGLNLFLWVGASVQQGVVQSLFSVSSFSQITSGLSVLPVLDNPLSKKVRGLIDSLRAQRSRYMKLTVVKQEDKMEMLFKHFLVEDKSLSGGASYVDFLCHMHKEIRQLLS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 SEC24 family, member C (S. cerevisiae)
别名 KIAA0079, SEC24 related protein C, SEC24C
计算分子量 1094 aa, 118 kDa
观测分子量 110-120 kDa
GenBank蛋白编号BC018928
基因名称 SEC24C
Gene ID (NCBI) 9632
RRIDAB_2186121
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP53992
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

COPII-coated vesicles mediate anterograde transport from the ER to the Golgi apparatus. COPII is composed of three parts: two coat protein complexes (Sec23/24 complex and Sec13/31 complex) and one small GTP-binding protein, Sar1 (PMID: 9568718). Sec24 subunit of the coat complex has been implicated in cargo recognition (PMID: 17316621). In mammal cells there are four Sec24 isoforms, termed Sec24A, Sec24B, Sec24C, and Sec24D (PMID: 10329445).

实验方案

Product Specific Protocols
WB protocol for SEC24C antibody 16073-1-APDownload protocol
IF protocol for SEC24C antibody 16073-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols