- Featured Product
- KD/KO Validated
SEC24A Polyclonal antibody
SEC24A Polyclonal Antibody for IF/ICC, IHC, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat, monkey
Applications
IF/ICC, IHC, WB,ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | COS-7 cells, HeLa cells, human liver tissue, mouse skeletal muscle tissue, mouse thymus tissue, rat thymus tissue, HEK-293 cells, HepG2 cells, NIH/3T3 cells |
| Positive IHC detected in | human lymphoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | Hela cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF) | IF : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
15958-1-AP targets SEC24A in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat, monkey samples.
| Tested Applications | IF/ICC, IHC, WB,ELISA |
| Cited Applications | WB, IP, IF |
| Tested Reactivity | human, mouse, rat, monkey |
| Cited Reactivity | human, mouse |
| Immunogen | SEC24A fusion protein Ag8723 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | SEC24 family, member A (S. cerevisiae) |
| Synonyms | SEC24 related protein A, SEC24A |
| Calculated Molecular Weight | 613 aa, 66 kDa |
| Observed Molecular Weight | 66 kDa, 120 kDa |
| GenBank Accession Number | BC019341 |
| Gene Symbol | SEC24A |
| Gene ID (NCBI) | 10802 |
| RRID | AB_2186118 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O95486 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
COPII-coated vesicles mediate anterograde transport from the ER to the Golgi apparatus. COPII is composed of three parts: two coat protein complexes (Sec23/24 complex and Sec13/31 complex) and one small GTP-binding protein, Sar1 (PMID: 9568718). Sec24 subunit of the coat complex has been implicated in cargo recognition (PMID: 17316621). In mammal cells there are four Sec24 isoforms, termed Sec24A, Sec24B, Sec24C, and Sec24D (PMID: 10329445). Two isoforms of 120 kDa and 66 kDa produced by alternative splicing have been described.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SEC24A antibody 15958-1-AP | Download protocol |
| IHC protocol for SEC24A antibody 15958-1-AP | Download protocol |
| IF protocol for SEC24A antibody 15958-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nature The CREB coactivator CRTC2 controls hepatic lipid metabolism by regulating SREBP1. | ||
J Clin Invest Sec13 promotes oligodendrocyte differentiation and myelin repair through autocrine pleiotrophin signaling. | ||
Redox Biol Selenoprotein K contributes to CD36 subcellular trafficking in hepatocytes by accelerating nascent COPII vesicle formation and aggravates hepatic steatosis | ||
J Immunother Cancer THADA drives Golgi residency and upregulation of PD-L1 in cancer cells and provides promising target for immunotherapy.
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