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SEC16B Polyclonal antibody

SEC16B Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 17245-1-AP

Print datasheet

Synonyms

KIAA1928, LZTR2, PGPR p117, RGPR, RGPR p117, SEC16 homolog B, SEC16B, SEC16S

验证数据展示

  • WB analysis using 17245-1-AP

    WB analysis using 17245-1-AP

    mouse pancreas tissue was subjected to SDS PAGE followed by western blot with 17245-1-AP (SEC16B antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas cancer using 17245-1-AP

    IHC staining of human pancreas cancer using 17245-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 17245-1-AP (SEC16B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse pancreas tissue
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17245-1-AP targets SEC16B in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IHC, WB, ELISA
Tested Reactivity human, mouse
Immunogen SEC16B fusion protein Ag11173 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name SEC16 homolog B (S. cerevisiae)
Synonyms KIAA1928, LZTR2, PGPR p117, RGPR, RGPR p117, SEC16 homolog B, SEC16B, SEC16S
Calculated Molecular Weight 71 kDa, 117 kDa
Observed Molecular Weight 60-70 kDa
GenBank Accession NumberBC009106
Gene Symbol SEC16B
Gene ID (NCBI) 89866
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96JE7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SEC16B also known as LZTR2, RGPR-p117, is a mammalian homolog of S. cerevisiae Sec16 that is required for organization of transitional endoplasmic reticulum (ER) sites and protein export (PMID: 17192411). SEC16B regulates the transport of peroxisomal biogenesis factors PEX3 and PEX16 from the ER to peroxisomes (PMID:21768384). SEC16B may be involved in human obesity to possess an association between single nucleotide polymorphisms and different measures of obesity (PMID: 28413634).

实验方案

Product Specific Protocols
WB protocol for SEC16B antibody 17245-1-APDownload protocol
IHC protocol for SEC16B antibody 17245-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols