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验证数据展示

  • FC experiment of HeLa using 29417-1-AP

    FC experiment of HeLa using 29417-1-AP

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human SEC16A (29417-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • WB analysis using 29417-1-AP

    WB analysis using 29417-1-AP

    Various lysates were subjected to Tris-acetate gel system followed by western blot with 29417-1-AP (SEC16A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse pancreas using 29417-1-AP

    IHC staining of mouse pancreas using 29417-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 29417-1-AP (SEC16A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SEC16A antibody included
  • IF Staining of HeLa using 29417-1-AP

    IF Staining of HeLa using 29417-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SEC16A antibody (29417-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

SEC16A Polyclonal antibody

SEC16A Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 29417-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, FC (Intra), ELISA

别名

SEC16L, SEC16 homolog A, SEC16, RP11 413M3.10, p250

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, HepG2 cells, HeLa cells
Positive IHC detected inmouse pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy SEC16A Ready-To-Use IHC Kit. SEC16A primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29417-1-AP targets SEC16A in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human, mouse
免疫原 SEC16A fusion protein Ag30700 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 SEC16 homolog A (S. cerevisiae)
别名 SEC16L, SEC16 homolog A, SEC16, RP11 413M3.10, p250
计算分子量 252 kDa
观测分子量 250-300 kDa
GenBank蛋白编号NM_014866
基因名称 SEC16A
Gene ID (NCBI) 9919
RRIDAB_3086130
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO15027
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SEC16A, also named as KIAA0310, SEC16 and SEC16L, is required for secretory cargo trafic from the endoplasmic reticulum to the Golgi apparatus. SAR1A-GTP-dependent assembly of SEC16A on the ER membrane forms an organized scaffold defining an ERES. SEC16A is required for normal transitional endoplasmic reticulum (tER) organization.

实验方案

Product Specific Protocols
WB protocol for SEC16A antibody 29417-1-APDownload protocol
IHC protocol for SEC16A antibody 29417-1-APDownload protocol
IF protocol for SEC16A antibody 29417-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols