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验证数据展示

  • IF Staining of A431 using 20025-1-AP (same clone as 20025-1-PBS)

    IF Staining of A431 using 20025-1-AP (same clone as 20025-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using SEC16A antibody (20025-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 20025-1-PBS in a different storage buffer formulation.

  • FC experiment of HEK-293 using 20025-1-AP (same clone as 20025-1-PBS)

    FC experiment of HEK-293 using 20025-1-AP (same clone as 20025-1-PBS)

    1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug Anti-Human SEC16A (20025-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 20025-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 20025-1-AP (same clone as 20025-1-PBS)

    IP experiment of HeLa using 20025-1-AP (same clone as 20025-1-PBS)

    IP result of anti-SEC16A (IP:20025-1-AP, 4ug; Detection:20025-1-AP 1:2000) with HeLa cells lysate 840 ug. This data was developed using the same antibody clone with 20025-1-PBS in a different storage buffer formulation.

  • WB analysis using 20025-1-AP (same clone as 20025-1-PBS)

    WB analysis using 20025-1-AP (same clone as 20025-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 20025-1-AP (SEC16A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20025-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse pancreas using 20025-1-AP (same clone as 20025-1-PBS)

    IHC staining of mouse pancreas using 20025-1-AP (same clone as 20025-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 20025-1-AP (SEC16A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20025-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SEC16A antibody included
  • IHC staining of mouse pancreas using 20025-1-AP (same clone as 20025-1-PBS)

    IHC staining of mouse pancreas using 20025-1-AP (same clone as 20025-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 20025-1-AP (SEC16A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20025-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SEC16A antibody included
  • IHC staining of mouse pancreas using 20025-1-AP (same clone as 20025-1-PBS)

    IHC staining of mouse pancreas using 20025-1-AP (same clone as 20025-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 20025-1-AP (SEC16A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20025-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SEC16A antibody included
  • Featured Product
  • KD/KO Validated

SEC16A Polyclonal antibody, PBS Only

SEC16A Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA
Cat No. 20025-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

别名

SEC16L, SEC16 homolog A, SEC16, RP11 413M3.10, p250

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy SEC16A Ready-To-Use IHC Kit. SEC16A primary antibody included.

产品信息

20025-1-PBS targets SEC16A in WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 SEC16 homolog A (S. cerevisiae)
别名 SEC16L, SEC16 homolog A, SEC16, RP11 413M3.10, p250
计算分子量 234 kDa
观测分子量 250-300 kDa
GenBank蛋白编号NM_014866
基因名称 SEC16A
Gene ID (NCBI) 9919
RRIDAB_2878635
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO15027
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SEC16A, also named as KIAA0310, SEC16 and SEC16L, is required for secretory cargo trafic from the endoplasmic reticulum to the Golgi apparatus. SAR1A-GTP-dependent assembly of SEC16A on the ER membrane forms an organized scaffold defining an ERES. SEC16A is required for normal transitional endoplasmic reticulum (tER) organization.

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