验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, mouse kidney tissue, rat kidney tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
16901-1-AP targets SEC11C in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 | SEC11C fusion protein Ag10442 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | SEC11 homolog C (S. cerevisiae) |
| 别名 | EC:3.4.21.89, SEC11 homolog C, SEC11 like protein 3, SEC11L3, SEC11-like protein 3 |
| 计算分子量 | 192 aa, 22 kDa |
| 观测分子量 | 16-22 kDa |
| GenBank蛋白编号 | BC009703 |
| 基因名称 | SEC11C |
| Gene ID (NCBI) | 90701 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9BY50 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SEC11C (Signal peptidase complex catalytic subunit SEC11C), also known as SPC21. It is located in the Endoplasmic reticulum membrane. Catalytic component of the signal peptidase complex (SPC) which catalyzes the cleavage of N-terminal signal sequences from nascent proteins as they are translocated into the lumen of the endoplasmic reticulum 1. Specifically cleaves N-terminal signal peptides that contain a hydrophobic alpha-helix (h-region) shorter than 18-20 amino acids. The molecular weight of SEC11C is 22 kDa. It has been reported that its molecular weight is 16kDa (PMID: 38183983).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SEC11C antibody 16901-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |